Synthesis of alpha 2-macroglobulin by bovine adrenal cortical cell cultures.

Published

Journal Article

Primary cultures of bovine adrenal medullary cells synthesize and secrete a high-molecular-weight protein into the culture medium. The protein was purified from the serum-free medium of cultured cells and was identified as alpha 2-macroglobulin by gel electrophoresis, sedimentation velocity, electron microscopy, immunoprecipitation, immunodiffusion, and autoradiography. Antisera directed against the protein were prepared and used to determine the cell types that synthesize the protein. Immunohistofluorescence studies show that adrenal cortical cells present in the adrenal medullary cell cultures reacted with the antisera to the protein purified from the medium, but adrenal medullary chromaffin cells did not. Cell cultures prepared from bovine adrenal cortex also synthesize and secrete alpha 2-macroglobulin and react with the antisera.

Full Text

Duke Authors

Cited Authors

  • Kirshner, N; Corcoran, JJ; Erickson, HP

Published Date

  • April 1989

Published In

Volume / Issue

  • 256 / 4 Pt 1

Start / End Page

  • C779 - C785

PubMed ID

  • 2468288

Pubmed Central ID

  • 2468288

Electronic International Standard Serial Number (EISSN)

  • 2163-5773

International Standard Serial Number (ISSN)

  • 0002-9513

Digital Object Identifier (DOI)

  • 10.1152/ajpcell.1989.256.4.c779

Language

  • eng