Synthesis of alpha 2-macroglobulin by bovine adrenal cortical cell cultures.
Primary cultures of bovine adrenal medullary cells synthesize and secrete a high-molecular-weight protein into the culture medium. The protein was purified from the serum-free medium of cultured cells and was identified as alpha 2-macroglobulin by gel electrophoresis, sedimentation velocity, electron microscopy, immunoprecipitation, immunodiffusion, and autoradiography. Antisera directed against the protein were prepared and used to determine the cell types that synthesize the protein. Immunohistofluorescence studies show that adrenal cortical cells present in the adrenal medullary cell cultures reacted with the antisera to the protein purified from the medium, but adrenal medullary chromaffin cells did not. Cell cultures prepared from bovine adrenal cortex also synthesize and secrete alpha 2-macroglobulin and react with the antisera.
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Related Subject Headings
- alpha-Macroglobulins
- Microscopy, Electron
- Kinetics
- Immunosorbent Techniques
- Histocytochemistry
- Fluorescent Antibody Technique
- Electrophoresis, Polyacrylamide Gel
- Chromatography
- Chromaffin System
- Centrifugation, Density Gradient
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- alpha-Macroglobulins
- Microscopy, Electron
- Kinetics
- Immunosorbent Techniques
- Histocytochemistry
- Fluorescent Antibody Technique
- Electrophoresis, Polyacrylamide Gel
- Chromatography
- Chromaffin System
- Centrifugation, Density Gradient