Tenascin-C knockout mouse has no detectable tenascin-C protein.

Journal Article

A recent study by Mitrovic and Schachner (J Neurosci Res 42:710-717, 1995) reported the detection of a small amount of truncated tensacin-C (TN-C) in the nervous system of the TN-C knockout mice created by Saga et al. (Genes Dev 6:1821-1831, 1992). The authors suggested that the truncated protein might be responsible for the failure to detect any phenotypic abnormalities in the knockout mice. We have reexamined the knockout mice in our laboratories by Western blot and immunocytochemistry, and have not detected any full-length or truncated TN-C protein. In addition, we note that the construction of the knockout gene deleted the signal sequence, so if any residual truncated protein were produced it would be trapped in the cytoplasm, and therefore inaccessible to extracellular ligands or receptors. We therefore conclude that the TN-C knockout created by Saga et al. is a valid TN-C null.

Full Text

Duke Authors

Cited Authors

  • Settles, DL; Kusakabe, M; Steindler, DA; Fillmore, H; Erickson, HP

Published Date

  • January 1, 1997

Published In

Volume / Issue

  • 47 / 1

Start / End Page

  • 109 - 117

PubMed ID

  • 8981244

International Standard Serial Number (ISSN)

  • 0360-4012

Language

  • eng

Conference Location

  • United States