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Visualization of purified fibronectin-transglutaminase complexes.

Publication ,  Journal Article
LeMosy, EK; Erickson, HP; Beyer, WF; Radek, JT; Jeong, JM; Murthy, SN; Lorand, L
Published in: J Biol Chem
April 15, 1992

It has been reported previously (Turner, P.M., and Lorand, L. (1989) Biochemistry 28, 628-635) that human erythrocyte transglutaminase forms a noncovalent complex with human plasma fibronectin near its collagen-binding domain. In the present study, we show by nondenaturing electrophoresis that guinea pig liver transglutaminase, similarly to the erythrocyte enzyme, forms a complex with human fibronectin. Studies of anisotropic shifts of fluorescein-labeled liver and erythrocyte transglutaminases, upon addition of fibronectin, indicated that both transglutaminases bind to fibronectin with a stoichiometry of about 2:1. Polymerization of fibrinogen by human erythrocyte transglutaminase was inhibited after complex formation with fibronectin. Complexes of fibronectin with either erythrocyte or liver transglutaminase were isolated by glycerol gradient zone sedimentation and examined by rotary shadowing electron microscopy. The globular transglutaminase could be readily identified binding to the thin fibronectin strand. The binding site for transglutaminase was within 5-10 nm of the N terminus of fibronectin, consistent with its proximity to the collagen-binding domain. Under some experimental conditions, the complex of fibronectin with erythrocyte transglutaminase appeared as a ring-shaped structure in which two transglutaminase molecules had probably dimerized. The molecular weight of the erythrocyte transglutaminase was determined by sedimentation equilibrium to be 71,440 +/- 830.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 15, 1992

Volume

267

Issue

11

Start / End Page

7880 / 7885

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transglutaminases
  • Species Specificity
  • Silver Staining
  • Microscopy, Electron
  • Liver
  • Humans
  • Guinea Pigs
  • Fluorescence Polarization
  • Fibronectins
 

Citation

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LeMosy, E. K., Erickson, H. P., Beyer, W. F., Radek, J. T., Jeong, J. M., Murthy, S. N., & Lorand, L. (1992). Visualization of purified fibronectin-transglutaminase complexes. J Biol Chem, 267(11), 7880–7885.
LeMosy, E. K., H. P. Erickson, W. F. Beyer, J. T. Radek, J. M. Jeong, S. N. Murthy, and L. Lorand. “Visualization of purified fibronectin-transglutaminase complexes.J Biol Chem 267, no. 11 (April 15, 1992): 7880–85.
LeMosy EK, Erickson HP, Beyer WF, Radek JT, Jeong JM, Murthy SN, et al. Visualization of purified fibronectin-transglutaminase complexes. J Biol Chem. 1992 Apr 15;267(11):7880–5.
LeMosy, E. K., et al. “Visualization of purified fibronectin-transglutaminase complexes.J Biol Chem, vol. 267, no. 11, Apr. 1992, pp. 7880–85.
LeMosy EK, Erickson HP, Beyer WF, Radek JT, Jeong JM, Murthy SN, Lorand L. Visualization of purified fibronectin-transglutaminase complexes. J Biol Chem. 1992 Apr 15;267(11):7880–7885.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 15, 1992

Volume

267

Issue

11

Start / End Page

7880 / 7885

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transglutaminases
  • Species Specificity
  • Silver Staining
  • Microscopy, Electron
  • Liver
  • Humans
  • Guinea Pigs
  • Fluorescence Polarization
  • Fibronectins