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Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins.

Publication ,  Journal Article
Anderson, DE; Gueiros-Filho, FJ; Erickson, HP
Published in: J Bacteriol
September 2004

FtsZ is the major cytoskeletal component of the bacterial cell division machinery. It forms a ring-shaped structure (the Z ring) that constricts as the bacterium divides. Previous in vivo experiments with green fluorescent protein-labeled FtsZ and fluorescence recovery after photobleaching have shown that the Escherichia coli Z ring is extremely dynamic, continually remodeling itself with a half time of 30 s, similar to microtubules in the mitotic spindle. In the present work, under different experimental conditions, we have found that the half time for fluorescence recovery of E. coli Z rings is even shorter (approximately 9 s). As before, the turnover appears to be coupled to GTP hydrolysis, since the mutant FtsZ84 protein, with reduced GTPase in vitro, showed an approximately 3-fold longer half time. We have also extended the studies to Bacillus subtilis and found that this species exhibits equally rapid dynamics of the Z ring (half time, approximately 8 s). Interestingly, null mutations of the FtsZ-regulating proteins ZapA, EzrA, and MinCD had only modest effects on the assembly dynamics. This suggests that these proteins do not directly regulate FtsZ subunit exchange in and out of polymers. In B. subtilis, only 30 to 35% of the FtsZ protein was in the Z ring, from which we conclude that a Z ring only 2 or 3 protofilaments thick can function for cell division.

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Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

September 2004

Volume

186

Issue

17

Start / End Page

5775 / 5781

Location

United States

Related Subject Headings

  • Mutation
  • Microbiology
  • Membrane Proteins
  • Luminescent Proteins
  • Half-Life
  • Guanosine Triphosphate
  • Green Fluorescent Proteins
  • Genes, Reporter
  • Genes, Bacterial
  • Gene Deletion
 

Citation

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Anderson, D. E., Gueiros-Filho, F. J., & Erickson, H. P. (2004). Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins. J Bacteriol, 186(17), 5775–5781. https://doi.org/10.1128/JB.186.17.5775-5781.2004
Anderson, David E., Frederico J. Gueiros-Filho, and Harold P. Erickson. “Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins.J Bacteriol 186, no. 17 (September 2004): 5775–81. https://doi.org/10.1128/JB.186.17.5775-5781.2004.
Anderson DE, Gueiros-Filho FJ, Erickson HP. Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins. J Bacteriol. 2004 Sep;186(17):5775–81.
Anderson, David E., et al. “Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins.J Bacteriol, vol. 186, no. 17, Sept. 2004, pp. 5775–81. Pubmed, doi:10.1128/JB.186.17.5775-5781.2004.
Anderson DE, Gueiros-Filho FJ, Erickson HP. Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins. J Bacteriol. 2004 Sep;186(17):5775–5781.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

September 2004

Volume

186

Issue

17

Start / End Page

5775 / 5781

Location

United States

Related Subject Headings

  • Mutation
  • Microbiology
  • Membrane Proteins
  • Luminescent Proteins
  • Half-Life
  • Guanosine Triphosphate
  • Green Fluorescent Proteins
  • Genes, Reporter
  • Genes, Bacterial
  • Gene Deletion