Skip to main content

In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB.

Publication ,  Journal Article
Sontag, CA; Staley, JT; Erickson, HP
Published in: J Cell Biol
April 25, 2005

Arecent study identified genuine tubulin proteins, BtubA and BtubB, in the bacterial genus Prosthecobacter. We have expressed BtubA and BtubB in Escherichia coli and studied their in vitro assembly. BtubB by itself formed rings with an outer diameter of 35-36 nm in the presence of GTP or GDP. Mixtures of BtubB and BtubA formed long protofilament bundles, 4-7 protofilaments wide (20-30 protofilaments in the three-dimensional bundle). Regardless of the starting stoichiometry, the polymers always contained equal concentrations of BtubA and BtubB, suggesting that BtubA and B alternate along the protofilament. BtubA showed negligible GTP hydrolysis, whereas BtubB hydrolyzed 0.40 mol GTP per min per mol BtubB. This GTPase activity increased to 1.37 per min when mixed 1:1 with BtubA. A critical concentration of 0.4-1.0 microM was indicated by light scattering experiments and extrapolation of GTPase versus concentration, thus suggesting a cooperative assembly mechanism.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

April 25, 2005

Volume

169

Issue

2

Start / End Page

233 / 238

Location

United States

Related Subject Headings

  • Tubulin
  • Multiprotein Complexes
  • Hydrolysis
  • Guanosine Triphosphate
  • Gram-Negative Bacteria
  • Developmental Biology
  • Bacterial Proteins
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 11 Medical and Health Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sontag, C. A., Staley, J. T., & Erickson, H. P. (2005). In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB. J Cell Biol, 169(2), 233–238. https://doi.org/10.1083/jcb.200410027
Sontag, Christopher A., James T. Staley, and Harold P. Erickson. “In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB.J Cell Biol 169, no. 2 (April 25, 2005): 233–38. https://doi.org/10.1083/jcb.200410027.
Sontag CA, Staley JT, Erickson HP. In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB. J Cell Biol. 2005 Apr 25;169(2):233–8.
Sontag, Christopher A., et al. “In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB.J Cell Biol, vol. 169, no. 2, Apr. 2005, pp. 233–38. Pubmed, doi:10.1083/jcb.200410027.
Sontag CA, Staley JT, Erickson HP. In vitro assembly and GTP hydrolysis by bacterial tubulins BtubA and BtubB. J Cell Biol. 2005 Apr 25;169(2):233–238.

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

April 25, 2005

Volume

169

Issue

2

Start / End Page

233 / 238

Location

United States

Related Subject Headings

  • Tubulin
  • Multiprotein Complexes
  • Hydrolysis
  • Guanosine Triphosphate
  • Gram-Negative Bacteria
  • Developmental Biology
  • Bacterial Proteins
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 11 Medical and Health Sciences