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Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissues.

Publication ,  Journal Article
Moioli, EK; Clark, PA; Chen, M; Dennis, JE; Erickson, HP; Gerson, SL; Mao, JJ
Published in: PloS one
January 2008

Poor angiogenesis is a major road block for tissue repair. The regeneration of virtually all tissues is limited by angiogenesis, given the diffusion of nutrients, oxygen, and waste products is limited to a few hundred micrometers. We postulated that co-transplantation of hematopoietic and mesenchymal stem/progenitor cells improves angiogenesis of tissue repair and hence the outcome of regeneration. In this study, we tested this hypothesis by using bone as a model whose regeneration is impaired unless it is vascularized. Hematopoietic stem/progenitor cells (HSCs) and mesenchymal stem/progenitor cells (MSCs) were isolated from each of three healthy human bone marrow samples and reconstituted in a porous scaffold. MSCs were seeded in micropores of 3D calcium phosphate (CP) scaffolds, followed by infusion of gel-suspended CD34(+) hematopoietic cells. Co-transplantation of CD34(+) HSCs and CD34(-) MSCs in microporous CP scaffolds subcutaneously in the dorsum of immunocompromised mice yielded vascularized tissue. The average vascular number of co-transplanted CD34(+) and MSC scaffolds was substantially greater than MSC transplantation alone. Human osteocalcin was expressed in the micropores of CP scaffolds and was significantly increased upon co-transplantation of MSCs and CD34(+) cells. Human nuclear staining revealed the engraftment of transplanted human cells in vascular endothelium upon co-transplantation of MSCs and CD34(+) cells. Based on additional in vitro results of endothelial differentiation of CD34(+) cells by vascular endothelial growth factor (VEGF), we adsorbed VEGF with co-transplanted CD34(+) and MSCs in the microporous CP scaffolds in vivo, and discovered that vascular number and diameter further increased, likely owing to the promotion of endothelial differentiation of CD34(+) cells by VEGF. Together, co-transplantation of hematopoietic and mesenchymal stem/progenitor cells may improve the regeneration of vascular dependent tissues such as bone, adipose, muscle and dermal grafts, and may have implications in the regeneration of internal organs.

Duke Scholars

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Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2008

Volume

3

Issue

12

Start / End Page

e3922

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Tissue Scaffolds
  • Tissue Engineering
  • Osteocalcin
  • Neovascularization, Physiologic
  • Mice, Nude
  • Mice
  • Mesenchymal Stem Cells
  • Mesenchymal Stem Cell Transplantation
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Moioli, E. K., Clark, P. A., Chen, M., Dennis, J. E., Erickson, H. P., Gerson, S. L., & Mao, J. J. (2008). Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissues. PloS One, 3(12), e3922. https://doi.org/10.1371/journal.pone.0003922
Moioli, Eduardo K., Paul A. Clark, Mo Chen, James E. Dennis, Helaman P. Erickson, Stanton L. Gerson, and Jeremy J. Mao. “Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissues.PloS One 3, no. 12 (January 2008): e3922. https://doi.org/10.1371/journal.pone.0003922.
Moioli EK, Clark PA, Chen M, Dennis JE, Erickson HP, Gerson SL, et al. Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissues. PloS one. 2008 Jan;3(12):e3922.
Moioli, Eduardo K., et al. “Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissues.PloS One, vol. 3, no. 12, Jan. 2008, p. e3922. Epmc, doi:10.1371/journal.pone.0003922.
Moioli EK, Clark PA, Chen M, Dennis JE, Erickson HP, Gerson SL, Mao JJ. Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissues. PloS one. 2008 Jan;3(12):e3922.

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2008

Volume

3

Issue

12

Start / End Page

e3922

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Tissue Scaffolds
  • Tissue Engineering
  • Osteocalcin
  • Neovascularization, Physiologic
  • Mice, Nude
  • Mice
  • Mesenchymal Stem Cells
  • Mesenchymal Stem Cell Transplantation
  • Male