Reconstitution of contractile FtsZ rings in liposomes.

Published

Journal Article

FtsZ is a tubulin homolog and the major cytoskeletal protein in bacterial cell division. It assembles into the Z ring, which contains FtsZ and a dozen other division proteins, and constricts to divide the cell. We have constructed a membrane-targeted FtsZ (FtsZ-mts) by splicing an amphipathic helix to its C terminus. When mixed with lipid vesicles, FtsZ-mts was incorporated into the interior of some tubular vesicles. There it formed multiple Z rings that could move laterally in both directions along the length of the liposome and coalesce into brighter Z rings. Brighter Z rings produced visible constrictions in the liposome, suggesting that FtsZ itself can assemble the Z ring and generate a force. No other proteins were needed for assembly and force generation.

Full Text

Duke Authors

Cited Authors

  • Osawa, M; Anderson, DE; Erickson, HP

Published Date

  • May 2008

Published In

Volume / Issue

  • 320 / 5877

Start / End Page

  • 792 - 794

PubMed ID

  • 18420899

Pubmed Central ID

  • 18420899

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1154520

Language

  • eng