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Assembly dynamics of Mycobacterium tuberculosis FtsZ.

Publication ,  Journal Article
Chen, Y; Anderson, DE; Rajagopalan, M; Erickson, HP
Published in: J Biol Chem
September 21, 2007

We have investigated the assembly of FtsZ from Mycobacterium tuberculosis (MtbFtsZ). Electron microscopy confirmed the previous observation that MtbFtsZ assembled into long, two-stranded filaments at pH 6.5. However, we found that assembly at pH 7.2 or 7.7 produced predominantly short, one-stranded protofilaments, similar to those of Escherichia coli FtsZ (EcFtsZ). Near pH 7, which is close to the pH of M. tuberculosis cytoplasm, MtbFtsZ formed a mixture of single- and two-stranded filaments. We developed a fluorescence resonance energy transfer assay to measure the kinetics of initial assembly and the dynamic properties at steady state. Assembly of MtbFtsZ reached a plateau after 60-100 s, about 10 times slower than EcFtsZ. The initial assembly kinetics were similar at pH 6.5 and 7.7, despite the striking difference in the polymer structures. Both were fit with a cooperative assembly mechanism involving a weak dimer nucleus, similar to EcFtsZ but with slower kinetics. Subunit turnover and GTPase at steady state were also about 10 times slower for MtbFtsZ than for EcFtsZ. Specifically, the half-time for subunit turnover in vitro at pH 7.7 was 42 s for MtbFtsZ compared with 5.5 s for EcFtsZ. Photobleaching studies in vivo showed a range of turnover half-times with an average of 25 s for MtbFtsZ as compared with 9 s for EcFtsZ.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

September 21, 2007

Volume

282

Issue

38

Start / End Page

27736 / 27743

Location

United States

Related Subject Headings

  • Time Factors
  • Polymers
  • Mycobacterium tuberculosis
  • Models, Chemical
  • Microscopy, Electron
  • Kinetics
  • Hydrogen-Ion Concentration
  • Guanosine Triphosphate
  • Guanosine Diphosphate
  • Fluorescence Resonance Energy Transfer
 

Citation

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Chen, Y., Anderson, D. E., Rajagopalan, M., & Erickson, H. P. (2007). Assembly dynamics of Mycobacterium tuberculosis FtsZ. J Biol Chem, 282(38), 27736–27743. https://doi.org/10.1074/jbc.M703788200
Chen, Yaodong, David E. Anderson, Malini Rajagopalan, and Harold P. Erickson. “Assembly dynamics of Mycobacterium tuberculosis FtsZ.J Biol Chem 282, no. 38 (September 21, 2007): 27736–43. https://doi.org/10.1074/jbc.M703788200.
Chen Y, Anderson DE, Rajagopalan M, Erickson HP. Assembly dynamics of Mycobacterium tuberculosis FtsZ. J Biol Chem. 2007 Sep 21;282(38):27736–43.
Chen, Yaodong, et al. “Assembly dynamics of Mycobacterium tuberculosis FtsZ.J Biol Chem, vol. 282, no. 38, Sept. 2007, pp. 27736–43. Pubmed, doi:10.1074/jbc.M703788200.
Chen Y, Anderson DE, Rajagopalan M, Erickson HP. Assembly dynamics of Mycobacterium tuberculosis FtsZ. J Biol Chem. 2007 Sep 21;282(38):27736–27743.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

September 21, 2007

Volume

282

Issue

38

Start / End Page

27736 / 27743

Location

United States

Related Subject Headings

  • Time Factors
  • Polymers
  • Mycobacterium tuberculosis
  • Models, Chemical
  • Microscopy, Electron
  • Kinetics
  • Hydrogen-Ion Concentration
  • Guanosine Triphosphate
  • Guanosine Diphosphate
  • Fluorescence Resonance Energy Transfer