Analysis of the interaction of primate retroviruses with the human RNA interference machinery.


Journal Article

The question of whether retroviruses, including human immunodeficiency virus type 1 (HIV-1), interact with the cellular RNA interference machinery has been controversial. Here, we present data showing that neither HIV-1 nor human T-cell leukemia virus type 1 (HTLV-1) expresses significant levels of either small interfering RNAs or microRNAs in persistently infected T cells. We also demonstrate that the retroviral nuclear transcription factors HIV-1 Tat and HTLV-1 Tax, as well as the Tas transactivator encoded by primate foamy virus, fail to inhibit RNA interference in human cells. Moreover, the stable expression of physiological levels of HIV-1 Tat did not globally inhibit microRNA production or expression in infected human cells. Together, these data argue that HIV-1 and HTLV-1 neither induce the production of viral small interfering RNAs or microRNAs nor repress the cellular RNA interference machinery in infected cells.

Full Text

Duke Authors

Cited Authors

  • Lin, J; Cullen, BR

Published Date

  • November 2007

Published In

Volume / Issue

  • 81 / 22

Start / End Page

  • 12218 - 12226

PubMed ID

  • 17855543

Pubmed Central ID

  • 17855543

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/JVI.01390-07


  • eng

Conference Location

  • United States