Induction of stable RNA interference in mammalian cells.

Journal Article (Journal Article;Review)

Over the last years, RNA interference (RNAi) has become a widely used technique that permits the knock-down, and hence functional analysis, of individual genes in vertebrate cells. However, the high failure rate of the RNA molecules used in RNAi experiments continues to be a problem. In this paper, I describe a set of design criteria, experimental steps and expression vectors that can facilitate the effective knock-down of almost any vertebrate gene product in cultured cells or in experimental animals.Gene Therapy (2006) 13, 503-508. doi:10.1038/; published online 29 September 2005.

Full Text

Duke Authors

Cited Authors

  • Cullen, BR

Published Date

  • March 2006

Published In

Volume / Issue

  • 13 / 6

Start / End Page

  • 503 - 508

PubMed ID

  • 16195700

International Standard Serial Number (ISSN)

  • 0969-7128

Digital Object Identifier (DOI)

  • 10.1038/


  • eng

Conference Location

  • England