Enhancing and confirming the specificity of RNAi experiments.

Journal Article (Journal Article;Review)

RNA interference (RNAi) provides a powerful technique for the derivation and analysis of loss-of-function phenotypes in vertebrate cells, where alternative approaches are either arduous or frequently ineffective. RNAi, however, is not always totally specific, and confirming the specificity, and hence the validity, of data obtained using RNAi therefore forms an essential component of experiments that rely on this technique. Here I briefly review the potential pitfalls associated with RNAi, and then suggest experimental approaches that can be used to maximize the specificity of RNAi or to confirm that the data obtained are indeed valid.

Full Text

Duke Authors

Cited Authors

  • Cullen, BR

Published Date

  • September 2006

Published In

Volume / Issue

  • 3 / 9

Start / End Page

  • 677 - 681

PubMed ID

  • 16929311

International Standard Serial Number (ISSN)

  • 1548-7091

Digital Object Identifier (DOI)

  • 10.1038/nmeth913


  • eng

Conference Location

  • United States