Use of RNA polymerase II to transcribe artificial microRNAs.

Journal Article (Journal Article)

MicroRNAs (miRNAs) are endogenously encoded approximately 22-nt-long RNAs that are generally expressed in a highly tissue- or developmental-stage-specific fashion and that posttranscriptionally regulate target genes. Regulatable RNA polymerase II promoters can be used to overexpress authentic microRNAs in cell culture. Furthermore, one can also design and express artificial microRNAs based on the features of existing microRNA genes, such as the gene encoding the human miR-30 microRNA. Overexpression or inappropriate expression of authentic microRNAs may facilitate the study of their normal functions and expression of artificial microRNAs may permit effective, regulated RNA interference in vivo.

Full Text

Duke Authors

Cited Authors

  • Zeng, Y; Cai, X; Cullen, BR

Published Date

  • 2005

Published In

Volume / Issue

  • 392 /

Start / End Page

  • 371 - 380

PubMed ID

  • 15644193

International Standard Serial Number (ISSN)

  • 0076-6879

Digital Object Identifier (DOI)

  • 10.1016/S0076-6879(04)92022-8


  • eng

Conference Location

  • United States