Selective inhibition of Alu retrotransposition by APOBEC3G.

Journal Article (Journal Article)

The non-LTR retrotransposon LINE-1 (L1) comprises approximately 17% of the human genome, and the L1-encoded proteins can function in trans to mediate the retrotransposition of non-autonomous retrotransposons (i.e., Alu and probably SVA elements) and cellular mRNAs to generate processed pseudogenes. Here, we have examined the effect of APOBEC3G and APOBEC3F, cytidine deaminases that inhibit Vif-deficient HIV-1 replication, on Alu retrotransposition and other L1-mediated retrotransposition processes. We demonstrate that APOBEC3G selectively inhibits Alu retrotransposition in an ORF1p-independent manner. An active cytidine deaminase site is not required for the inhibition of Alu retrotransposition and the resultant integration events lack G to A or C to T hypermutation. These data demonstrate a differential restriction of L1 and Alu retrotransposition by APOBEC3G, and suggest that the Alu ribonucleoprotein complex may be targeted by APOBEC3G.

Full Text

Duke Authors

Cited Authors

  • Hulme, AE; Bogerd, HP; Cullen, BR; Moran, JV

Published Date

  • April 1, 2007

Published In

Volume / Issue

  • 390 / 1-2

Start / End Page

  • 199 - 205

PubMed ID

  • 17079095

Pubmed Central ID

  • PMC2917221

International Standard Serial Number (ISSN)

  • 0378-1119

Digital Object Identifier (DOI)

  • 10.1016/j.gene.2006.08.032


  • eng

Conference Location

  • Netherlands