Selective inhibition of Alu retrotransposition by APOBEC3G.
Journal Article (Journal Article)
The non-LTR retrotransposon LINE-1 (L1) comprises approximately 17% of the human genome, and the L1-encoded proteins can function in trans to mediate the retrotransposition of non-autonomous retrotransposons (i.e., Alu and probably SVA elements) and cellular mRNAs to generate processed pseudogenes. Here, we have examined the effect of APOBEC3G and APOBEC3F, cytidine deaminases that inhibit Vif-deficient HIV-1 replication, on Alu retrotransposition and other L1-mediated retrotransposition processes. We demonstrate that APOBEC3G selectively inhibits Alu retrotransposition in an ORF1p-independent manner. An active cytidine deaminase site is not required for the inhibition of Alu retrotransposition and the resultant integration events lack G to A or C to T hypermutation. These data demonstrate a differential restriction of L1 and Alu retrotransposition by APOBEC3G, and suggest that the Alu ribonucleoprotein complex may be targeted by APOBEC3G.
Full Text
Duke Authors
Cited Authors
- Hulme, AE; Bogerd, HP; Cullen, BR; Moran, JV
Published Date
- April 1, 2007
Published In
Volume / Issue
- 390 / 1-2
Start / End Page
- 199 - 205
PubMed ID
- 17079095
Pubmed Central ID
- PMC2917221
International Standard Serial Number (ISSN)
- 0378-1119
Digital Object Identifier (DOI)
- 10.1016/j.gene.2006.08.032
Language
- eng
Conference Location
- Netherlands