Specific packaging of APOBEC3G into HIV-1 virions is mediated by the nucleocapsid domain of the gag polyprotein precursor.
Journal Article (Journal Article)
In cells infected by HIV-1 mutants lacking a functional Vif protein, APOBEC3G is specifically packaged into progeny virions and then interferes with the process of virus infection. Here, we show that incorporation of APOBEC3G into HIV-1 virions is mediated by the specific interaction of APOBEC3G with the carboxy-terminal nucleocapsid/p6 domain of the Gag polyprotein precursor. As a result, HIV-1 virus-like particles that lack the nucleocapsid domain fail to package APOBEC3G. Surprisingly, RNA was also found to be essential for formation of the nucleocapsid--APOBEC3G complex in vitro, thus raising the possibility that RNA may form a bridge between these two proteins.
Full Text
Duke Authors
Cited Authors
- Schäfer, A; Bogerd, HP; Cullen, BR
Published Date
- October 25, 2004
Published In
Volume / Issue
- 328 / 2
Start / End Page
- 163 - 168
PubMed ID
- 15464836
International Standard Serial Number (ISSN)
- 0042-6822
Digital Object Identifier (DOI)
- 10.1016/j.virol.2004.08.006
Language
- eng
Conference Location
- United States