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In vivo models for studying mast cell-dependent responses to bacterial infection.

Publication ,  Journal Article
Shelburne, CP; McLachlan, JB; Abraham, SN
Published in: Methods Mol Biol
2006

Mast cells are a critical component of host defense against bacterial infections. Activation of these cells during infection induces both innate and adaptive aspects of protective immunity needed for the elimination of the bacteria and survival of the host. These functional roles for the mast cell have been principally characterized using two in vivo models of acute bacterial infection featuring Gram-negative pathogens such as Escherichia coli. Here, we present basic protocols for the identification of mast cell-dependent biological functions during bacterial infection. These include the use of mast cell-deficient mice, the identification of mast cells in tissue, the culture of uropathogenic E. coli, and the basic analysis of mast cell-dependent functions in the peritoneal cavity and footpad models of bacterial pathogenesis.

Duke Scholars

Published In

Methods Mol Biol

DOI

ISSN

1064-3745

Publication Date

2006

Volume

315

Start / End Page

363 / 381

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Peritoneal Cavity
  • Mice, Inbred Strains
  • Mice
  • Mast Cells
  • Lymphocytes
  • Lymph Nodes
  • Immune System
  • Foot
  • Escherichia coli
 

Citation

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ICMJE
MLA
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Shelburne, C. P., McLachlan, J. B., & Abraham, S. N. (2006). In vivo models for studying mast cell-dependent responses to bacterial infection. Methods Mol Biol, 315, 363–381. https://doi.org/10.1385/1-59259-967-2:363
Shelburne, Christopher P., James B. McLachlan, and Soman N. Abraham. “In vivo models for studying mast cell-dependent responses to bacterial infection.Methods Mol Biol 315 (2006): 363–81. https://doi.org/10.1385/1-59259-967-2:363.
Shelburne CP, McLachlan JB, Abraham SN. In vivo models for studying mast cell-dependent responses to bacterial infection. Methods Mol Biol. 2006;315:363–81.
Shelburne, Christopher P., et al. “In vivo models for studying mast cell-dependent responses to bacterial infection.Methods Mol Biol, vol. 315, 2006, pp. 363–81. Pubmed, doi:10.1385/1-59259-967-2:363.
Shelburne CP, McLachlan JB, Abraham SN. In vivo models for studying mast cell-dependent responses to bacterial infection. Methods Mol Biol. 2006;315:363–381.

Published In

Methods Mol Biol

DOI

ISSN

1064-3745

Publication Date

2006

Volume

315

Start / End Page

363 / 381

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Peritoneal Cavity
  • Mice, Inbred Strains
  • Mice
  • Mast Cells
  • Lymphocytes
  • Lymph Nodes
  • Immune System
  • Foot
  • Escherichia coli