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Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation.

Publication ,  Journal Article
Malherbe, DC; Erpenbeck, VJ; Abraham, SN; Crouch, EC; Hohlfeld, JM; Wright, JR
Published in: Am J Physiol Lung Cell Mol Physiol
November 2005

Mast cells play a key role in allergy and asthma. They reside at the host-environment interface and are among the first cells to make contact with inhaled microorganisms and particulate antigens. Pulmonary surfactant proteins A and D (SP-A and SP-D) function in lung host defense by enhancing microbe phagocytosis and mediating other immune cell functions, but little is known about their effects on mast cells. We hypothesized that SP-A and/or SP-D modulate IgE-dependent mast cell functions. Pollen starch granules (PSG) extracted from Dactylis glomerata and coated with trinitrophenol (TNP) were used as a model of an inhaled organic particulate allergen. Our data revealed that SP-D inhibited by 50% the release of beta-hexosaminidase by peritoneal mast cells sensitized with IgE anti-TNP and stimulated with TNP-PSG. In contrast, SP-A had no effect. Furthermore, SP-D aggregated PSG in a dose-dependent manner, and this aggregation was mediated by SP-D's carbohydrate recognition domain. A single arm SP-D mutant (RrSP-Dser15,20) neither aggregated PSG nor inhibited degranulation, suggesting that multimerization of SP-D is required for maximal PSG aggregation and inhibition of PSG-induced mast cell degranulation. This study is the first to demonstrate that SP-D modulates IgE-mediated mast cell functions, which are important in asthma and allergic inflammation.

Duke Scholars

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

November 2005

Volume

289

Issue

5

Start / End Page

L856 / L866

Location

United States

Related Subject Headings

  • Respiratory System
  • Recombinant Proteins
  • Rats
  • Pulmonary Surfactant-Associated Protein D
  • Pulmonary Surfactant-Associated Protein A
  • Protein Structure, Quaternary
  • Pollen
  • Mice
  • Mast Cells
  • Immunoglobulin E
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Malherbe, D. C., Erpenbeck, V. J., Abraham, S. N., Crouch, E. C., Hohlfeld, J. M., & Wright, J. R. (2005). Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation. Am J Physiol Lung Cell Mol Physiol, 289(5), L856–L866. https://doi.org/10.1152/ajplung.00009.2005
Malherbe, Delphine C., Veit J. Erpenbeck, Soman N. Abraham, Erika C. Crouch, Jens M. Hohlfeld, and Jo Rae Wright. “Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation.Am J Physiol Lung Cell Mol Physiol 289, no. 5 (November 2005): L856–66. https://doi.org/10.1152/ajplung.00009.2005.
Malherbe DC, Erpenbeck VJ, Abraham SN, Crouch EC, Hohlfeld JM, Wright JR. Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation. Am J Physiol Lung Cell Mol Physiol. 2005 Nov;289(5):L856–66.
Malherbe, Delphine C., et al. “Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation.Am J Physiol Lung Cell Mol Physiol, vol. 289, no. 5, Nov. 2005, pp. L856–66. Pubmed, doi:10.1152/ajplung.00009.2005.
Malherbe DC, Erpenbeck VJ, Abraham SN, Crouch EC, Hohlfeld JM, Wright JR. Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation. Am J Physiol Lung Cell Mol Physiol. 2005 Nov;289(5):L856–L866.

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

November 2005

Volume

289

Issue

5

Start / End Page

L856 / L866

Location

United States

Related Subject Headings

  • Respiratory System
  • Recombinant Proteins
  • Rats
  • Pulmonary Surfactant-Associated Protein D
  • Pulmonary Surfactant-Associated Protein A
  • Protein Structure, Quaternary
  • Pollen
  • Mice
  • Mast Cells
  • Immunoglobulin E