Purification of outer membrane vesicles from Pseudomonas aeruginosa and their activation of an IL-8 response.

Journal Article (Journal Article)

Considerable lung injury results from the inflammatory response to Pseudomonas aeruginosa infections in patients with cystic fibrosis (CF). The P. aeruginosa laboratory strain PAO1, an environmental isolate, and isolates from CF patients were cultured in vitro and outer membrane vesicles from those cultures were quantitated, purified, and characterized. Vesicles were produced throughout the growth phases of the culture and vesicle yield was strain-independent. Strain-dependent differences in the protein composition of vesicles were quantitated and identified. The aminopeptidase PaAP (PA2939) was highly enriched in vesicles from CF isolates. Vesicles from all strains elicited IL-8 secretion by lung epithelial cells. These results suggest that P. aeruginosa colonizing the CF lung may produce vesicles with a particular composition and that the vesicles could contribute to inflammation.

Full Text

Duke Authors

Cited Authors

  • Bauman, SJ; Kuehn, MJ

Published Date

  • August 2006

Published In

Volume / Issue

  • 8 / 9-10

Start / End Page

  • 2400 - 2408

PubMed ID

  • 16807039

Pubmed Central ID

  • PMC3525494

International Standard Serial Number (ISSN)

  • 1286-4579

Digital Object Identifier (DOI)

  • 10.1016/j.micinf.2006.05.001


  • eng

Conference Location

  • France