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Identification and characterization of adrenergic receptors and catecholamine-stimulated adenylate cyclase in hog pial membranes.

Publication ,  Journal Article
Friedman, AH; Davis, JN
Published in: Brain Res
February 3, 1980

In order to define the adrenergic receptors in small pial blood vessels, we studied [3H]dihydroalprenolol (DHA) binding, [3H]dihydroergocryptine (DHE) binding, and catecholamine-stimulated adenylate cyclase in membranes prepared from the hog pia. [3H]dha bound to a saturable number of sites (0.09 pmol/mg protein) with a high affinity to (10 mM). The ability of a small series of adrenergic agents to compete for [3H]DHA binding was as expected for binding to beta-adrenergic membrane receptors. The receptor binding appeared to be composed largely of beta 2-adrenergic sites but some sites with beta 1 properties were detected. The beta-adrenergic agonist, isoproterenol, stimulated adenylate cyclase activity by 20% in pial membranes in the presence of GTP. The potency of catecholamines in stimulating adenylate cyclase correlated well with theif sites (0.39 pmol/mg) on these membranes. alpha-Adrenergic agents were potent competitors for [3H]DHE binding but dopamine, serotonin and histamine had effects only at high concentrations. These data demonstrate the presence of alpha- and beta-adrenergic membrane receptors and beta-adrenergically stimulated adenylate cyclase activity in small pial blood vessels from the hog.

Duke Scholars

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

February 3, 1980

Volume

183

Issue

1

Start / End Page

89 / 102

Location

Netherlands

Related Subject Headings

  • Swine
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic, alpha
  • Receptors, Adrenergic
  • Radioligand Assay
  • Pia Mater
  • Neurology & Neurosurgery
  • Dihydroergotoxine
  • Dihydroalprenolol
  • Cerebral Cortex
 

Citation

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Friedman, A. H., & Davis, J. N. (1980). Identification and characterization of adrenergic receptors and catecholamine-stimulated adenylate cyclase in hog pial membranes. Brain Res, 183(1), 89–102. https://doi.org/10.1016/0006-8993(80)90121-3
Friedman, A. H., and J. N. Davis. “Identification and characterization of adrenergic receptors and catecholamine-stimulated adenylate cyclase in hog pial membranes.Brain Res 183, no. 1 (February 3, 1980): 89–102. https://doi.org/10.1016/0006-8993(80)90121-3.
Friedman, A. H., and J. N. Davis. “Identification and characterization of adrenergic receptors and catecholamine-stimulated adenylate cyclase in hog pial membranes.Brain Res, vol. 183, no. 1, Feb. 1980, pp. 89–102. Pubmed, doi:10.1016/0006-8993(80)90121-3.
Journal cover image

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

February 3, 1980

Volume

183

Issue

1

Start / End Page

89 / 102

Location

Netherlands

Related Subject Headings

  • Swine
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic, alpha
  • Receptors, Adrenergic
  • Radioligand Assay
  • Pia Mater
  • Neurology & Neurosurgery
  • Dihydroergotoxine
  • Dihydroalprenolol
  • Cerebral Cortex