Transcription factor E2-2 is an essential and specific regulator of plasmacytoid dendritic cell development.

Published

Journal Article

Plasmacytoid dendritic cells (PDCs) represent a unique immune cell type specialized in type I interferon (IFN) secretion in response to viral nucleic acids. The molecular control of PDC lineage specification has been poorly understood. We report that basic helix-loop-helix transcription factor (E protein) E2-2/Tcf4 is preferentially expressed in murine and human PDCs. Constitutive or inducible deletion of murine E2-2 blocked the development of PDCs but not of other lineages and abolished IFN response to unmethylated DNA. Moreover, E2-2 haploinsufficiency in mice and in human Pitt-Hopkins syndrome patients was associated with aberrant expression profile and impaired IFN response of the PDC. E2-2 directly activated multiple PDC-enriched genes, including transcription factors involved in PDC development (SpiB, Irf8) and function (Irf7). These results identify E2-2 as a specific transcriptional regulator of the PDC lineage in mice and humans and reveal a key function of E proteins in the innate immune system.

Full Text

Duke Authors

Cited Authors

  • Cisse, B; Caton, ML; Lehner, M; Maeda, T; Scheu, S; Locksley, R; Holmberg, D; Zweier, C; den Hollander, NS; Kant, SG; Holter, W; Rauch, A; Zhuang, Y; Reizis, B

Published Date

  • October 3, 2008

Published In

Volume / Issue

  • 135 / 1

Start / End Page

  • 37 - 48

PubMed ID

  • 18854153

Pubmed Central ID

  • 18854153

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2008.09.016

Language

  • eng

Conference Location

  • United States