A large-scale functional approach to uncover human genes and pathways in Drosophila.

Journal Article

We demonstrate the feasibility of performing a systematic screen for human gene functions in Drosophila by assaying for their ability to induce overexpression phenotypes. Over 1 500 transgenic fly lines corresponding to 236 human genes have been established. In all, 51 lines are capable of eliciting a phenotype suggesting that the human genes are functional. These heterologous genes are functionally relevant as we have found a similar mutant phenotype caused either by a dominant negative mutant form of the human ribosomal protein L8 gene or by RNAi downregulation of the Drosophila RPL8. Significantly, the Drosophila RPL8 mutant can be rescued by wild-type human RPL8. We also provide genetic evidence that Drosophila RPL8 is a new member of the insulin signaling pathway. In summary, the functions of many human genes appear to be highly conserved, and the ability to identify them in Drosophila represents a powerful genetic tool for large-scale analysis of human transcripts in vivo.

Full Text

Duke Authors

Cited Authors

  • Xu, R; Deng, K; Zhu, Y; Wu, Y; Ren, J; Wan, M; Zhao, S; Wu, X; Han, M; Zhuang, Y; Xu, T

Published Date

  • November 2008

Published In

Volume / Issue

  • 18 / 11

Start / End Page

  • 1114 - 1127

PubMed ID

  • 18957936

Pubmed Central ID

  • 18957936

Electronic International Standard Serial Number (EISSN)

  • 1748-7838

Digital Object Identifier (DOI)

  • 10.1038/cr.2008.295


  • eng

Conference Location

  • England