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Receptor-selective coactivators as tools to define the biology of specific receptor-coactivator pairs.

Publication ,  Journal Article
Gaillard, S; Grasfeder, LL; Haeffele, CL; Lobenhofer, EK; Chu, T-M; Wolfinger, R; Kazmin, D; Koves, TR; Muoio, DM; Chang, C-Y; McDonnell, DP
Published in: Mol Cell
December 8, 2006

In the absence of specific high-affinity agonists and antagonists, it has been difficult to define the target genes and biological responses attributable to many of the orphan nuclear receptors (ONRs). Indeed, it appears that many members of this receptor superfamily are not regulated by classical small molecules but rather their activity is controlled by interacting cofactors. Motivated by this finding, we have developed an approach to genetically isolate specific receptor-cofactor pairs in cells, allowing us to define the biological responses attributable to each complex. This is accomplished by using combinatorial peptide phage display to engineer the receptor interacting domain of each cofactor such that it interacts selectively with one nuclear receptor. In this study, we describe the customization of PGC-1alpha and its use to study the biology of the estrogen-related receptor alpha (ERRalpha) in cultured liver cells.

Duke Scholars

Published In

Mol Cell

DOI

ISSN

1097-2765

Publication Date

December 8, 2006

Volume

24

Issue

5

Start / End Page

797 / 803

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription Factors
  • Receptors, Estrogen
  • RNA, Small Interfering
  • RNA, Messenger
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
  • Peptide Library
  • Oxidation-Reduction
  • Humans
  • Hepatocytes
 

Citation

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MLA
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Gaillard, S., Grasfeder, L. L., Haeffele, C. L., Lobenhofer, E. K., Chu, T.-M., Wolfinger, R., … McDonnell, D. P. (2006). Receptor-selective coactivators as tools to define the biology of specific receptor-coactivator pairs. Mol Cell, 24(5), 797–803. https://doi.org/10.1016/j.molcel.2006.10.012
Gaillard, Stéphanie, Linda L. Grasfeder, Christiane L. Haeffele, Edward K. Lobenhofer, Tzu-Ming Chu, Russ Wolfinger, Dmitri Kazmin, et al. “Receptor-selective coactivators as tools to define the biology of specific receptor-coactivator pairs.Mol Cell 24, no. 5 (December 8, 2006): 797–803. https://doi.org/10.1016/j.molcel.2006.10.012.
Gaillard S, Grasfeder LL, Haeffele CL, Lobenhofer EK, Chu T-M, Wolfinger R, et al. Receptor-selective coactivators as tools to define the biology of specific receptor-coactivator pairs. Mol Cell. 2006 Dec 8;24(5):797–803.
Gaillard, Stéphanie, et al. “Receptor-selective coactivators as tools to define the biology of specific receptor-coactivator pairs.Mol Cell, vol. 24, no. 5, Dec. 2006, pp. 797–803. Pubmed, doi:10.1016/j.molcel.2006.10.012.
Gaillard S, Grasfeder LL, Haeffele CL, Lobenhofer EK, Chu T-M, Wolfinger R, Kazmin D, Koves TR, Muoio DM, Chang C-Y, McDonnell DP. Receptor-selective coactivators as tools to define the biology of specific receptor-coactivator pairs. Mol Cell. 2006 Dec 8;24(5):797–803.
Journal cover image

Published In

Mol Cell

DOI

ISSN

1097-2765

Publication Date

December 8, 2006

Volume

24

Issue

5

Start / End Page

797 / 803

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription Factors
  • Receptors, Estrogen
  • RNA, Small Interfering
  • RNA, Messenger
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
  • Peptide Library
  • Oxidation-Reduction
  • Humans
  • Hepatocytes