Receptor-selective coactivators as tools to define the biology of specific receptor-coactivator pairs.
Published
Journal Article
In the absence of specific high-affinity agonists and antagonists, it has been difficult to define the target genes and biological responses attributable to many of the orphan nuclear receptors (ONRs). Indeed, it appears that many members of this receptor superfamily are not regulated by classical small molecules but rather their activity is controlled by interacting cofactors. Motivated by this finding, we have developed an approach to genetically isolate specific receptor-cofactor pairs in cells, allowing us to define the biological responses attributable to each complex. This is accomplished by using combinatorial peptide phage display to engineer the receptor interacting domain of each cofactor such that it interacts selectively with one nuclear receptor. In this study, we describe the customization of PGC-1alpha and its use to study the biology of the estrogen-related receptor alpha (ERRalpha) in cultured liver cells.
Full Text
Duke Authors
- Chang, Ching-yi
- Gaillard, Stephanie
- Koves, Timothy Robert
- McDonnell, Donald Patrick
- Muoio, Deborah Marie
Cited Authors
- Gaillard, S; Grasfeder, LL; Haeffele, CL; Lobenhofer, EK; Chu, T-M; Wolfinger, R; Kazmin, D; Koves, TR; Muoio, DM; Chang, C-Y; McDonnell, DP
Published Date
- December 8, 2006
Published In
Volume / Issue
- 24 / 5
Start / End Page
- 797 - 803
PubMed ID
- 17157261
Pubmed Central ID
- 17157261
International Standard Serial Number (ISSN)
- 1097-2765
Digital Object Identifier (DOI)
- 10.1016/j.molcel.2006.10.012
Language
- eng
Conference Location
- United States