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High-resolution magnetic resonance histology of the embryonic and neonatal mouse: a 4D atlas and morphologic database.

Publication ,  Journal Article
Petiet, AE; Kaufman, MH; Goddeeris, MM; Brandenburg, J; Elmore, SA; Johnson, GA
Published in: Proc Natl Acad Sci U S A
August 26, 2008

Engineered mice play an ever-increasing role in defining connections between genotype and phenotypic expression. The potential of magnetic resonance microscopy (MRM) for morphologic phenotyping in the mouse has previously been demonstrated; however, applications have been limited by long scan times, availability of the technology, and a foundation of normative data. This article describes an integrated environment for high-resolution study of normal, transgenic, and mutant mouse models at embryonic and neonatal stages. Three-dimensional images are shown at an isotropic resolution of 19.5 microm (voxel volumes of 8 pL), acquired in 3 h at embryonic days 10.5-19.5 (10 stages) and postnatal days 0-32 (6 stages). A web-accessible atlas encompassing this data was developed, and for critical stages of embryonic development (prenatal days 14.5-18.5), >200 anatomical structures have been identified and labeled. Also, matching optical histology and analysis tools are provided to compare multiple specimens at multiple developmental stages. The utility of the approach is demonstrated in characterizing cardiac septal defects in conditional mutant embryos lacking the Smoothened receptor gene. Finally, a collaborative paradigm is presented that allows sharing of data across the scientific community. This work makes magnetic resonance microscopy of the mouse embryo and neonate broadly available with carefully annotated normative data and an extensive environment for collaborations.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

August 26, 2008

Volume

105

Issue

34

Start / End Page

12331 / 12336

Location

United States

Related Subject Headings

  • Time Factors
  • Microscopy
  • Mice, Transgenic
  • Mice, Mutant Strains
  • Mice
  • Magnetic Resonance Imaging
  • Imaging, Three-Dimensional
  • Heart Septal Defects
  • Embryonic Development
  • Embryo, Mammalian
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Petiet, A. E., Kaufman, M. H., Goddeeris, M. M., Brandenburg, J., Elmore, S. A., & Johnson, G. A. (2008). High-resolution magnetic resonance histology of the embryonic and neonatal mouse: a 4D atlas and morphologic database. Proc Natl Acad Sci U S A, 105(34), 12331–12336. https://doi.org/10.1073/pnas.0805747105
Petiet, Alexandra E., Matthew H. Kaufman, Matthew M. Goddeeris, Jeffrey Brandenburg, Susan A. Elmore, and G Allan Johnson. “High-resolution magnetic resonance histology of the embryonic and neonatal mouse: a 4D atlas and morphologic database.Proc Natl Acad Sci U S A 105, no. 34 (August 26, 2008): 12331–36. https://doi.org/10.1073/pnas.0805747105.
Petiet AE, Kaufman MH, Goddeeris MM, Brandenburg J, Elmore SA, Johnson GA. High-resolution magnetic resonance histology of the embryonic and neonatal mouse: a 4D atlas and morphologic database. Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12331–6.
Petiet, Alexandra E., et al. “High-resolution magnetic resonance histology of the embryonic and neonatal mouse: a 4D atlas and morphologic database.Proc Natl Acad Sci U S A, vol. 105, no. 34, Aug. 2008, pp. 12331–36. Pubmed, doi:10.1073/pnas.0805747105.
Petiet AE, Kaufman MH, Goddeeris MM, Brandenburg J, Elmore SA, Johnson GA. High-resolution magnetic resonance histology of the embryonic and neonatal mouse: a 4D atlas and morphologic database. Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12331–12336.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

August 26, 2008

Volume

105

Issue

34

Start / End Page

12331 / 12336

Location

United States

Related Subject Headings

  • Time Factors
  • Microscopy
  • Mice, Transgenic
  • Mice, Mutant Strains
  • Mice
  • Magnetic Resonance Imaging
  • Imaging, Three-Dimensional
  • Heart Septal Defects
  • Embryonic Development
  • Embryo, Mammalian