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Enhanced T2 contrast for MR histology of the mouse brain.

Publication ,  Journal Article
Sharief, AA; Johnson, GA
Published in: Magn Reson Med
October 2006

A 3D Carr-Purcell-Meiboom-Gill (CPMG) sequence was implemented to obtain enhanced T(2) contrast in actively stained (perfusion with fixative and contrast agent) mouse brains at 9.4 T. Short interecho spacing was used to minimize diffusion and susceptibility losses. The sequence produced 16 3D volumes with an interecho spacing of 7 ms for isotropic 43-mu-resolution images of the mouse brains in a scan time of 4 hr. To enhance the signal-to-noise ratio (SNR) and contrast, the multiecho frequency domain image contrast (MEFIC) method was applied, resulting in a composite image with T(2)-weighted contrast. The high SNR and contrast thus achieved revealed aspects of mouse brain morphology, such as multiple cortical layers, groups of thalamic nuclei, layers of the inferior and superior colliculus, and molecular and granular layers of the cerebellum, with a high degree of definition and contrast that was not previously achieved in T(2)-weighted acquisitions at high fields.

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Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

October 2006

Volume

56

Issue

4

Start / End Page

717 / 725

Location

United States

Related Subject Headings

  • Staining and Labeling
  • Organometallic Compounds
  • Nuclear Medicine & Medical Imaging
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Magnetic Resonance Imaging
  • Least-Squares Analysis
  • In Vitro Techniques
  • Imaging, Three-Dimensional
 

Citation

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MLA
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Sharief, A. A., & Johnson, G. A. (2006). Enhanced T2 contrast for MR histology of the mouse brain. Magn Reson Med, 56(4), 717–725. https://doi.org/10.1002/mrm.21026
Sharief, Anjum Ali, and G Allan Johnson. “Enhanced T2 contrast for MR histology of the mouse brain.Magn Reson Med 56, no. 4 (October 2006): 717–25. https://doi.org/10.1002/mrm.21026.
Sharief AA, Johnson GA. Enhanced T2 contrast for MR histology of the mouse brain. Magn Reson Med. 2006 Oct;56(4):717–25.
Sharief, Anjum Ali, and G. Allan Johnson. “Enhanced T2 contrast for MR histology of the mouse brain.Magn Reson Med, vol. 56, no. 4, Oct. 2006, pp. 717–25. Pubmed, doi:10.1002/mrm.21026.
Sharief AA, Johnson GA. Enhanced T2 contrast for MR histology of the mouse brain. Magn Reson Med. 2006 Oct;56(4):717–725.
Journal cover image

Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

October 2006

Volume

56

Issue

4

Start / End Page

717 / 725

Location

United States

Related Subject Headings

  • Staining and Labeling
  • Organometallic Compounds
  • Nuclear Medicine & Medical Imaging
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Magnetic Resonance Imaging
  • Least-Squares Analysis
  • In Vitro Techniques
  • Imaging, Three-Dimensional