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A novel 2-oxoacid-dependent dioxygenase involved in the formation of the goiterogenic 2-hydroxybut-3-enyl glucosinolate and generalist insect resistance in Arabidopsis,.

Publication ,  Journal Article
Hansen, BG; Kerwin, RE; Ober, JA; Lambrix, VM; Mitchell-Olds, T; Gershenzon, J; Halkier, BA; Kliebenstein, DJ
Published in: Plant physiology
December 2008

Glucosinolates are secondary metabolites found almost exclusively in the order Brassicales. They are synthesized from a variety of amino acids and can have numerous side chain modifications that control biological function. We investigated the biosynthesis of 2-hydroxybut-3-enyl glucosinolate, which has biological activities including toxicity to Caenorhabditis elegans, inhibition of seed germination, induction of goiter disease in mammals, and production of bitter flavors in Brassica vegetable crops. Arabidopsis (Arabidopsis thaliana) accessions contain three different patterns of 2-hydroxybut-3-enyl glucosinolate accumulation (present in leaves and seeds, seeds only, or absent) corresponding to three different alleles at a single locus, GSL-OH. Fine-scale mapping of the GSL-OH locus identified a 2-oxoacid-dependent dioxygenase encoded by At2g25450 required for the formation of both 2R- and 2S-2-hydroxybut-3-enyl glucosinolate from the precursor 3-butenyl glucosinolate precursor. Naturally occurring null mutations and T-DNA insertional mutations in At2g25450 exhibit a complete absence of 2-hydroxybut-3-enyl glucosinolate accumulation. Analysis of herbivory by the generalist lepidopteran Trichoplusia ni showed that production of 2-hydroxybut-3-enyl glucosinolate provides increased resistance. These results show that At2g25450 is necessary for the hydroxylation of but-3-enyl glucosinolate to 2-hydroxybut-3-enyl glucosinolate in planta and that this metabolite increases resistance to generalist herbivory.

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Published In

Plant physiology

DOI

EISSN

1532-2548

ISSN

0032-0889

Publication Date

December 2008

Volume

148

Issue

4

Start / End Page

2096 / 2108

Related Subject Headings

  • Sequence Analysis, DNA
  • Promoter Regions, Genetic
  • Plant Biology & Botany
  • Mutagenesis, Insertional
  • Moths
  • Insecta
  • Glucosinolates
  • Genotype
  • Genetic Variation
  • Feeding Behavior
 

Citation

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Hansen, B. G., Kerwin, R. E., Ober, J. A., Lambrix, V. M., Mitchell-Olds, T., Gershenzon, J., … Kliebenstein, D. J. (2008). A novel 2-oxoacid-dependent dioxygenase involved in the formation of the goiterogenic 2-hydroxybut-3-enyl glucosinolate and generalist insect resistance in Arabidopsis,. Plant Physiology, 148(4), 2096–2108. https://doi.org/10.1104/pp.108.129981
Hansen, Bjarne G., Rachel E. Kerwin, James A. Ober, Virginia M. Lambrix, Thomas Mitchell-Olds, Jonathan Gershenzon, Barbara A. Halkier, and Daniel J. Kliebenstein. “A novel 2-oxoacid-dependent dioxygenase involved in the formation of the goiterogenic 2-hydroxybut-3-enyl glucosinolate and generalist insect resistance in Arabidopsis,.Plant Physiology 148, no. 4 (December 2008): 2096–2108. https://doi.org/10.1104/pp.108.129981.
Hansen BG, Kerwin RE, Ober JA, Lambrix VM, Mitchell-Olds T, Gershenzon J, et al. A novel 2-oxoacid-dependent dioxygenase involved in the formation of the goiterogenic 2-hydroxybut-3-enyl glucosinolate and generalist insect resistance in Arabidopsis,. Plant physiology. 2008 Dec;148(4):2096–108.
Hansen, Bjarne G., et al. “A novel 2-oxoacid-dependent dioxygenase involved in the formation of the goiterogenic 2-hydroxybut-3-enyl glucosinolate and generalist insect resistance in Arabidopsis,.Plant Physiology, vol. 148, no. 4, Dec. 2008, pp. 2096–108. Epmc, doi:10.1104/pp.108.129981.
Hansen BG, Kerwin RE, Ober JA, Lambrix VM, Mitchell-Olds T, Gershenzon J, Halkier BA, Kliebenstein DJ. A novel 2-oxoacid-dependent dioxygenase involved in the formation of the goiterogenic 2-hydroxybut-3-enyl glucosinolate and generalist insect resistance in Arabidopsis,. Plant physiology. 2008 Dec;148(4):2096–2108.

Published In

Plant physiology

DOI

EISSN

1532-2548

ISSN

0032-0889

Publication Date

December 2008

Volume

148

Issue

4

Start / End Page

2096 / 2108

Related Subject Headings

  • Sequence Analysis, DNA
  • Promoter Regions, Genetic
  • Plant Biology & Botany
  • Mutagenesis, Insertional
  • Moths
  • Insecta
  • Glucosinolates
  • Genotype
  • Genetic Variation
  • Feeding Behavior