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Analysis of ENU-induced mutations at the Adh locus in Drosophila melanogaster.

Publication ,  Journal Article
Fossett, NG; Arbour-Reily, P; Kilroy, G; McDaniel, M; Mahmoud, J; Tucker, AB; Chang, SH; Lee, WR
Published in: Mutat Res
July 1990

N-Ethyl-N-nitrosourea (ENU) was used to induce mutations in the Drosophila melanogaster, alcohol dehydrogenase (Adh) gene. Flies were treated with ENU and mated to homozygous intragenic Adh null mutants; Adh null mutations were selected by exposure of the F1 generation to 1-penten-3-ol. Fourteen Adh null mutations were recovered which included 11 from spermatozoa, 2 from oocytes and 1 from a premeiotic spermatocyte. 2 mutations from spermatozoa and 1 of the mutations from oocytes were multilocus deficiencies which included the Adh locus as determined by complementation tests. The remaining 11 intragenic Adh null mutations were sequenced using the Sanger dideoxy method. One Adh null mutation induced in an oocyte was an AT to TA transversion and the mutation induced in a premeiotic spermatocyte was a GC to AT transition, both of which resulted in a single amino acid substitution. The 11 null mutations induced in spermatozoa were a data set in which both the dose of ENU and the treated germ-cell stage were held constant; therefore, only these 11 mutations were used to calculate the mutation frequency and compare the mutations at the Adh locus with those recovered in other studies. The dose of ENU induced a sex-linked recessive lethal frequency approximately 300 times that of the spontaneous frequency; therefore, these mutations were assumed to have been induced by ENU. 2 of the 11 mutations induced in spermatozoa were multilocus deficiencies and 9 were intragenic mutations. 7 of the 9 intragenic mutations were GC to AT transitions which resulted in 5 single amino acid substitutions, 1 premature translation termination codon, and 1 splice site mutation.(ABSTRACT TRUNCATED AT 400 WORDS)

Duke Scholars

Published In

Mutat Res

DOI

ISSN

0027-5107

Publication Date

July 1990

Volume

231

Issue

1

Start / End Page

73 / 85

Location

Netherlands

Related Subject Headings

  • Restriction Mapping
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Mutation
  • Molecular Sequence Data
  • Male
  • Introns
  • Genetic Complementation Test
  • Genes
  • Female
 

Citation

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ICMJE
MLA
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Fossett, N. G., Arbour-Reily, P., Kilroy, G., McDaniel, M., Mahmoud, J., Tucker, A. B., … Lee, W. R. (1990). Analysis of ENU-induced mutations at the Adh locus in Drosophila melanogaster. Mutat Res, 231(1), 73–85. https://doi.org/10.1016/0027-5107(90)90178-7
Fossett, N. G., P. Arbour-Reily, G. Kilroy, M. McDaniel, J. Mahmoud, A. B. Tucker, S. H. Chang, and W. R. Lee. “Analysis of ENU-induced mutations at the Adh locus in Drosophila melanogaster.Mutat Res 231, no. 1 (July 1990): 73–85. https://doi.org/10.1016/0027-5107(90)90178-7.
Fossett NG, Arbour-Reily P, Kilroy G, McDaniel M, Mahmoud J, Tucker AB, et al. Analysis of ENU-induced mutations at the Adh locus in Drosophila melanogaster. Mutat Res. 1990 Jul;231(1):73–85.
Fossett, N. G., et al. “Analysis of ENU-induced mutations at the Adh locus in Drosophila melanogaster.Mutat Res, vol. 231, no. 1, July 1990, pp. 73–85. Pubmed, doi:10.1016/0027-5107(90)90178-7.
Fossett NG, Arbour-Reily P, Kilroy G, McDaniel M, Mahmoud J, Tucker AB, Chang SH, Lee WR. Analysis of ENU-induced mutations at the Adh locus in Drosophila melanogaster. Mutat Res. 1990 Jul;231(1):73–85.
Journal cover image

Published In

Mutat Res

DOI

ISSN

0027-5107

Publication Date

July 1990

Volume

231

Issue

1

Start / End Page

73 / 85

Location

Netherlands

Related Subject Headings

  • Restriction Mapping
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Mutation
  • Molecular Sequence Data
  • Male
  • Introns
  • Genetic Complementation Test
  • Genes
  • Female