Immunogold fine structural localization of extracellular matrix components in aged human cornea. I. Types I-IV collagen and laminin.

Journal Article

Using the immunogold technique combined with cryoultramicrotomy and London Resin white (LR white) embedding, we studied the fine structural distribution of types I-IV collagen and laminin in corneal tissue from seven enucleated human eyes (age range, 63-78 years). Type II collagen was not identified in any corneal layer. Type I and type III collagen were distributed in a similar fashion in striated collagen fibrils in Bowman's layer and in the stroma. Type IV collagen was located only in the posterior non-banded region of Descemet's membrane. Laminin was identified in subepithelial anchoring plaques and the sub-endothelial region of Descemet's membrane in accordance with its recognized adhesive function.

Full Text

Duke Authors

Cited Authors

  • Marshall, GE; Konstas, AG; Lee, WR

Published Date

  • January 1, 1991

Published In

Volume / Issue

  • 229 / 2

Start / End Page

  • 157 - 163

PubMed ID

  • 2044978

Pubmed Central ID

  • 2044978

Electronic International Standard Serial Number (EISSN)

  • 1435-702X

International Standard Serial Number (ISSN)

  • 0721-832X

Digital Object Identifier (DOI)

  • 10.1007/bf00170550

Language

  • eng