Immunogold fine structural localization of extracellular matrix components in aged human cornea. I. Types I-IV collagen and laminin.
Journal Article
Using the immunogold technique combined with cryoultramicrotomy and London Resin white (LR white) embedding, we studied the fine structural distribution of types I-IV collagen and laminin in corneal tissue from seven enucleated human eyes (age range, 63-78 years). Type II collagen was not identified in any corneal layer. Type I and type III collagen were distributed in a similar fashion in striated collagen fibrils in Bowman's layer and in the stroma. Type IV collagen was located only in the posterior non-banded region of Descemet's membrane. Laminin was identified in subepithelial anchoring plaques and the sub-endothelial region of Descemet's membrane in accordance with its recognized adhesive function.
Full Text
Duke Authors
Cited Authors
- Marshall, GE; Konstas, AG; Lee, WR
Published Date
- January 1, 1991
Published In
Volume / Issue
- 229 / 2
Start / End Page
- 157 - 163
PubMed ID
- 2044978
Pubmed Central ID
- 2044978
Electronic International Standard Serial Number (EISSN)
- 1435-702X
International Standard Serial Number (ISSN)
- 0721-832X
Digital Object Identifier (DOI)
- 10.1007/bf00170550
Language
- eng