Molecular analysis of Drosophila melanogaster AdhnLA405 confirms reliability of DNA-sequencing methodology.

Journal Article (Journal Article)

An alcohol dehydrogenase (ADH) null mutant of Drosophila melanogaster (AdhnLA405) originally recovered following X-ray irradiation of mature sperm (Aaron, 979) is analyzed by Southern blotting, Western blotting, and DNA sequencing. The genetic, immunologic, and nucleic acid sequence data are consistent with the hypothesis that a cross-over event, independent of X-irradiation, between parental chromosomes is responsible for the ADH null phenotype of AdhnLA405. By DNA-sequence analysis we show that molecular cloning of this locus (i.e., propagation in prokaryotic hosts) apparently does not introduce any spurious changes (substitutions, additions, deletions, or rearrangements) within the DNA.

Full Text

Duke Authors

Cited Authors

  • LoMonaco, MB; Batzer, MA; Tedeschi, B; Chang, SH; Lee, WR

Published Date

  • February 1, 1988

Published In

Volume / Issue

  • 207 / 2

Start / End Page

  • 53 - 56

PubMed ID

  • 3123925

International Standard Serial Number (ISSN)

  • 0027-5107

Digital Object Identifier (DOI)

  • 10.1016/0165-7992(88)90041-3

Language

  • eng

Conference Location

  • Netherlands