Pressure effects on flow channels in the lining endothelium of Schlemm's canal. A quantitative study by transmission electron microscopy.

Journal Article (Journal Article)

A quantitative investigation, using transmission electron microscopy, was conducted to determine the relative incidence of nuclei, giant vacuoles and their pores, non-vacuolar transcellular channels and minipores in the endothelium lining the trabecular aspect of Schlemm's canal at various levels of intraocular pressure in the range 0--50 mmHg. The eyes of twelve rhesus monkeys were used and prior to intracameral fixation, the intraocular pressure was maintained at the appropriate level for one hour. The results obtained from a single section analysis showed that the incidence of giant vacuoles, vacuolar and non-vacuolar transcellular channels and minipores could be related to pressure. An analysis of serial reconstructions of samples of the vacuoles indicated that vacuolar transcellular channels became more numerous and more enlarged as pressure was increased. We concluded that the transcellular pore system serves as a major outflow route for aqueous humour.

Full Text

Duke Authors

Cited Authors

  • Grierson, I; Lee, WR

Published Date

  • December 1, 1978

Published In

Volume / Issue

  • 56 / 6

Start / End Page

  • 935 - 952

PubMed ID

  • 103360

International Standard Serial Number (ISSN)

  • 0001-639X

Digital Object Identifier (DOI)

  • 10.1111/j.1755-3768.1978.tb03813.x


  • eng

Conference Location

  • Denmark