A simplified method for the isolation of highly purified bovine retinal S antigen.

Journal Article (Journal Article)

Retinal S antigen is a potent autoantigen used for the induction of experimental allergic uveoretinitis (EAU). EAU is an organ-specific disease and shows many similarities to other autoimmune diseases such as experimental encephalomyelitis. This paper describes the preparation of highly purified S antigen by using a one-step ion-exchange method. High yields of the protein were obtained. S antigen prepared by this method induces a prolonged posterior uveoretinitis with cellular infiltration in the vitreous and specific loss of retinal photoreceptor cells. The purity of the protein was checked by silver-stained SDS-polyacrylamide gels and immunoblotting techniques.

Full Text

Duke Authors

Cited Authors

  • Al-Mahdawi, S; Forrester, JV; Lee, WR

Published Date

  • February 1, 1987

Published In

Volume / Issue

  • 14 / 1

Start / End Page

  • 99 - 108

PubMed ID

  • 3805262

International Standard Serial Number (ISSN)

  • 0165-5728

Digital Object Identifier (DOI)

  • 10.1016/0165-5728(87)90104-4


  • eng

Conference Location

  • Netherlands