A simplified method for the isolation of highly purified bovine retinal S antigen.
Journal Article (Journal Article)
Retinal S antigen is a potent autoantigen used for the induction of experimental allergic uveoretinitis (EAU). EAU is an organ-specific disease and shows many similarities to other autoimmune diseases such as experimental encephalomyelitis. This paper describes the preparation of highly purified S antigen by using a one-step ion-exchange method. High yields of the protein were obtained. S antigen prepared by this method induces a prolonged posterior uveoretinitis with cellular infiltration in the vitreous and specific loss of retinal photoreceptor cells. The purity of the protein was checked by silver-stained SDS-polyacrylamide gels and immunoblotting techniques.
- Al-Mahdawi, S; Forrester, JV; Lee, WR
- February 1, 1987
Volume / Issue
- 14 / 1
Start / End Page
- 99 - 108
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)