The 40-kilodalton allergen of Candida albicans is an alcohol dehydrogenase: molecular cloning and immunological analysis using monoclonal antibodies.

Journal Article (Journal Article)

To characterize the 40-kilodalton (kD) major allergen of Candida albicans (C. albicans), six monoclonal antibodies (MoAbs) against this allergen were generated. In SDS-polyacrylamide gel electrophoresis and immunoblot analysis, these MoAbs showed four different reaction patterns to antigens of six different Candida species. With the exception of one MoAb, other MoAbs were resistant to periodate treatment indicating non-carbohydrate epitopes were probably being recognized by these MoAbs. These MoAbs were used in the molecular cloning and immunological analysis of the gene coding for the 40-kD allergen. Nucleotide sequence determination of the two lambda gt11 cDNA clones obtained showed that the 40-kD allergen is an alcohol dehydrogenase (ADH) which shares a 70% amino acid sequence homology with the ADH isozyme I of Saccharomyces cerevisiae. This finding was confirmed by positive immunological response of the lysates of the clones obtained and a preparation of ADH of Saccharomyces cerevisiae to various MoAbs and to IgE antibodies in sera of allergic patients.

Full Text

Duke Authors

Cited Authors

  • Shen, HD; Choo, KB; Lee, HH; Hsieh, JC; Lin, WL; Lee, WR; Han, SH

Published Date

  • November 1991

Published In

Volume / Issue

  • 21 / 6

Start / End Page

  • 675 - 681

PubMed ID

  • 1777830

Pubmed Central ID

  • 1777830

International Standard Serial Number (ISSN)

  • 0954-7894

Digital Object Identifier (DOI)

  • 10.1111/j.1365-2222.1991.tb03195.x

Language

  • eng

Conference Location

  • England