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In vitro efficacy of a polyhexamethylene biguanide-impregnated gauze dressing against bacteria found in veterinary patients.

Publication ,  Journal Article
Lee, WR; Tobias, KM; Bemis, DA; Rohrbach, BW
Published in: Vet Surg
2004

OBJECTIVE: To evaluate the in vitro efficacy of polyhexamethylene biguanide (PHMB)-impregnated gauze dressing in limiting the growth of bacteria both within and underneath the dressing. STUDY DESIGN: In vitro study. METHODS: Squares of PHMB-impregnated and control gauze were placed on agar plates inoculated with 1 of 11 bacterial species, including 8 multi-resistant organisms. Growth under the gauze was assessed qualitatively after 24-hour incubation. Repeated use of sponges was used to evaluate residual inhibitory activity against Micrococcus lutea and Staphylococcus schleiferi ss. schleiferi. In a second procedure, PHMB-impregnated and control gauze squares were placed in sterile plastic wells and inoculated with 1 of 5 bacterial species, including Pseudomonas spp. and Klebsiella spp. Inhibition of bacterial growth within and underneath the dressing after 24-hour incubation was evaluated by quantifying the numbers of bacteria on the well floor and within each square. RESULTS: PHMB-impregnated gauze provided greater inhibition of growth of 4/4 Gram-positive species and 2/6 Gram-negative species on inoculated plates compared with control gauze. Residual inhibitory activity of PHMB-impregnated gauze was significantly greater against M. lutea on all days and against S. schleiferi ss. schleiferi on days 1 and 4 compared with control. No bacteria were recovered from inoculated PHMB-impregnated gauze squares placed in sterile wells or from the well floor underneath. More than 9 x 10(5) colony-forming units (CFU) were recovered from inoculated control samples placed in sterile wells and more than 8.4 x 10(4) CFU were recovered from control well floors. CONCLUSION: PHMB-impregnated gauze dressing, when placed on inoculated agar plates, reduces growth of underlying bacteria, particularly Gram-positive species. Wet-inoculated PHMB-impregnated dressing prevents growth of Gram-positive and Gram-negative bacteria both within and underneath the dressing. CLINICAL RELEVANCE: PHMB-impregnated dressings may be useful for reducing contamination of underlying wounds by bacterial pathogens.

Duke Scholars

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Published In

Vet Surg

DOI

ISSN

0161-3499

Publication Date

2004

Volume

33

Issue

4

Start / End Page

404 / 411

Location

United States

Related Subject Headings

  • Wound Infection
  • Veterinary Sciences
  • Microbial Sensitivity Tests
  • Gram-Positive Bacteria
  • Gram-Negative Bacteria
  • Dogs
  • Dog Diseases
  • Disinfectants
  • Cats
  • Cat Diseases
 

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Lee, W. R., Tobias, K. M., Bemis, D. A., & Rohrbach, B. W. (2004). In vitro efficacy of a polyhexamethylene biguanide-impregnated gauze dressing against bacteria found in veterinary patients. Vet Surg, 33(4), 404–411. https://doi.org/10.1111/j.1532-950X.2004.04059.x
Lee, William R., Karen M. Tobias, David A. Bemis, and Barton W. Rohrbach. “In vitro efficacy of a polyhexamethylene biguanide-impregnated gauze dressing against bacteria found in veterinary patients.Vet Surg 33, no. 4 (2004): 404–11. https://doi.org/10.1111/j.1532-950X.2004.04059.x.
Lee, William R., et al. “In vitro efficacy of a polyhexamethylene biguanide-impregnated gauze dressing against bacteria found in veterinary patients.Vet Surg, vol. 33, no. 4, 2004, pp. 404–11. Pubmed, doi:10.1111/j.1532-950X.2004.04059.x.
Journal cover image

Published In

Vet Surg

DOI

ISSN

0161-3499

Publication Date

2004

Volume

33

Issue

4

Start / End Page

404 / 411

Location

United States

Related Subject Headings

  • Wound Infection
  • Veterinary Sciences
  • Microbial Sensitivity Tests
  • Gram-Positive Bacteria
  • Gram-Negative Bacteria
  • Dogs
  • Dog Diseases
  • Disinfectants
  • Cats
  • Cat Diseases