Synthesis of programmable integrases.
Journal Article (Journal Article)
Accurate modification of the 3 billion-base-pair human genome requires tools with exceptional sequence specificity. Here, we describe a general strategy for the design of enzymes that target a single site within the genome. We generated chimeric zinc finger recombinases with cooperative DNA-binding and catalytic specificities that integrate transgenes with >98% accuracy into the human genome. These modular recombinases can be reprogrammed: New combinations of zinc finger domains and serine recombinase catalytic domains generate novel enzymes with distinct substrate sequence specificities. Because of their accuracy and versatility, the recombinases/integrases reported in this work are suitable for a wide variety of applications in biological research, medicine, and biotechnology where accurate delivery of DNA is desired.
Full Text
Duke Authors
Cited Authors
- Gordley, RM; Gersbach, CA; Barbas, CF
Published Date
- March 12, 2009
Published In
Volume / Issue
- 106 / 13
Start / End Page
- 5053 - 5058
PubMed ID
- 19282480
Pubmed Central ID
- PMC2654808
Electronic International Standard Serial Number (EISSN)
- 1091-6490
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.0812502106
Language
- eng