Synthesis of programmable integrases.

Published

Journal Article

Accurate modification of the 3 billion-base-pair human genome requires tools with exceptional sequence specificity. Here, we describe a general strategy for the design of enzymes that target a single site within the genome. We generated chimeric zinc finger recombinases with cooperative DNA-binding and catalytic specificities that integrate transgenes with >98% accuracy into the human genome. These modular recombinases can be reprogrammed: New combinations of zinc finger domains and serine recombinase catalytic domains generate novel enzymes with distinct substrate sequence specificities. Because of their accuracy and versatility, the recombinases/integrases reported in this work are suitable for a wide variety of applications in biological research, medicine, and biotechnology where accurate delivery of DNA is desired.

Full Text

Duke Authors

Cited Authors

  • Gordley, RM; Gersbach, CA; Barbas, CF

Published Date

  • March 12, 2009

Published In

Volume / Issue

  • 106 / 13

Start / End Page

  • 5053 - 5058

PubMed ID

  • 19282480

Pubmed Central ID

  • 19282480

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0812502106

Language

  • eng