Caenorhabditis elegans-based screen identifies Salmonella virulence factors required for conserved host-pathogen interactions.

Published

Journal Article

A Caenorhabditis elegans-Salmonella enterica host-pathogen model was used to identify both novel and previously known S. enterica virulence factors (HilA, HilD, InvH, SptP, RhuM, Spi4-F, PipA, VsdA, RepC, Sb25, RfaL, GmhA, LeuO, CstA, and RecC), including several related to the type III secretion system (TTSS) encoded in Salmonella pathogenicity island 1 (SPI-1). Mutants corresponding to presumptive novel virulence-related genes exhibited diminished ability to invade epithelial cells and/or to induce polymorphonuclear leukocyte migration in a tissue culture model of mammalian enteropathogenesis. When expressed in C. elegans intestinal cells, the S. enterica TTSS-exported effector protein SptP inhibited a conserved p38 MAPK signaling pathway and suppressed the diminished pathogenicity phenotype of an S. enterica sptP mutant. These results show that C. elegans is an attractive model to study the interaction between Salmonella effector proteins and components of the innate immune response, in part because there is a remarkable overlap between Salmonella virulence factors required for human and nematode pathogenesis.

Full Text

Duke Authors

Cited Authors

  • Tenor, JL; McCormick, BA; Ausubel, FM; Aballay, A

Published Date

  • June 8, 2004

Published In

Volume / Issue

  • 14 / 11

Start / End Page

  • 1018 - 1024

PubMed ID

  • 15182677

Pubmed Central ID

  • 15182677

International Standard Serial Number (ISSN)

  • 0960-9822

Digital Object Identifier (DOI)

  • 10.1016/j.cub.2004.05.050

Language

  • eng

Conference Location

  • England