High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection.

Published

Journal Article

We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluorescent bacteria inside the animals. Furthermore, we validate the use of Amplifluor as a new single nucleotide polymorphism (SNP) mapping technique in C. elegans. We show that a set of 9 SNPs allows the linkage of C. elegans mutants to a 5-8 megabase sub-chromosomal region.

Full Text

Cited Authors

  • Fuhrman, LE; Shianna, KV; Aballay, A

Published Date

  • August 6, 2008

Published In

Volume / Issue

  • 3 / 8

Start / End Page

  • e2882 -

PubMed ID

  • 18682730

Pubmed Central ID

  • 18682730

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

International Standard Serial Number (ISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0002882

Language

  • eng