Skip to main content
Journal cover image

Pentoxifylline modulates deformability, F-actin content, and superoxide anion production of polymorphonuclear leukocytes from diabetic cats.

Publication ,  Journal Article
Sonkin, PL; Freedman, SF; Needham, D; Rao, KM; Hatchell, DL
Published in: Exp Eye Res
December 1992

Capillary occlusion is an early event in the development of diabetic retinopathy, and white blood cells have recently been shown to be involved. We have shown previously that pentoxifylline improves deformability and decreases F-actin content of unstimulated polymorphonuclear leukocytes from normal human subjects. The purpose of this study was to determine if pentoxifylline would improve three properties of unstimulated polymorphonuclear leukocytes from diabetic cats. The measured parameters were mechanical (whole cell deformability), structural (F-actin content) and biochemical (rate of superoxide anion production). Chronic hyperglycemia was induced in three cats by partial pancreatectomy, and they were kept in poor glycemic control for at least 6 months prior to the study. Polymorphonuclear leukocytes were isolated and the entry time of individual passive cells was measured during aspiration into a 4-micron micropipette under constant suction pressure (-15 cmH2O). Deformability was defined as the inverse of the entry time. F-actin content of passive cells was measured by NBD-phallacidin labeling followed by flow cytometry. The rate of superoxide anion production was measured spectrophotometrically by superoxide dismutase-inhibitable cytochrome c reduction. Following incubation for 15 min with 0.1, 1.0 and 10.0 mM pentoxifylline, the average entry time of passive polymorphonuclear leukocytes was reduced from control by 11 +/- 5% (P = 0.045), 17 +/- 6% (P = 0.007), and 36 +/- 5% (P < 0.001), respectively. The F-actin content decreased by 0%, 4 +/- 0.6% (P < 0.001), and 10 +/- 3% (P < 0.001), respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

December 1992

Volume

55

Issue

6

Start / End Page

831 / 838

Location

England

Related Subject Headings

  • Superoxides
  • Pentoxifylline
  • Ophthalmology & Optometry
  • Neutrophils
  • Disease Models, Animal
  • Diabetic Retinopathy
  • Cats
  • Anions
  • Animals
  • Actins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sonkin, P. L., Freedman, S. F., Needham, D., Rao, K. M., & Hatchell, D. L. (1992). Pentoxifylline modulates deformability, F-actin content, and superoxide anion production of polymorphonuclear leukocytes from diabetic cats. Exp Eye Res, 55(6), 831–838. https://doi.org/10.1016/0014-4835(92)90009-h
Sonkin, P. L., S. F. Freedman, D. Needham, K. M. Rao, and D. L. Hatchell. “Pentoxifylline modulates deformability, F-actin content, and superoxide anion production of polymorphonuclear leukocytes from diabetic cats.Exp Eye Res 55, no. 6 (December 1992): 831–38. https://doi.org/10.1016/0014-4835(92)90009-h.
Sonkin, P. L., et al. “Pentoxifylline modulates deformability, F-actin content, and superoxide anion production of polymorphonuclear leukocytes from diabetic cats.Exp Eye Res, vol. 55, no. 6, Dec. 1992, pp. 831–38. Pubmed, doi:10.1016/0014-4835(92)90009-h.
Sonkin PL, Freedman SF, Needham D, Rao KM, Hatchell DL. Pentoxifylline modulates deformability, F-actin content, and superoxide anion production of polymorphonuclear leukocytes from diabetic cats. Exp Eye Res. 1992 Dec;55(6):831–838.
Journal cover image

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

December 1992

Volume

55

Issue

6

Start / End Page

831 / 838

Location

England

Related Subject Headings

  • Superoxides
  • Pentoxifylline
  • Ophthalmology & Optometry
  • Neutrophils
  • Disease Models, Animal
  • Diabetic Retinopathy
  • Cats
  • Anions
  • Animals
  • Actins