Synthesis and biological evaluation of an 123I-labeled bicyclic nucleoside analogue (BCNA) as potential SPECT tracer for VZV-tk reporter gene imaging.

Published

Journal Article

An iodine-123 labeled bicyclic nucleoside analogue ([(123)I]-4) has been synthesized and evaluated as a potential single photon emission tomography (SPECT) reporter probe for the non-invasive imaging of expression of the varicella zoster virus thymidine kinase (VZV-tk) reporter gene. In vitro enzymatic assays revealed that the non-radioactive mono-iodo derivative 4 has good affinity for VZV-TK (IC(50): 4.2 microM). Biodistribution of [(123)I]-4 was examined in normal mice. Evaluation of [(123)I]-4 in HEK-293T cells showed 1.74-fold higher accumulation in VZV-TK-expressing cells compared to control cells.

Full Text

Duke Authors

Cited Authors

  • Chitneni, SK; Deroose, CM; Fonge, H; Gijsbers, R; Dyubankova, N; Balzarini, J; Debyser, Z; Mortelmans, L; Verbruggen, AM; Bormans, GM

Published Date

  • June 15, 2007

Published In

Volume / Issue

  • 17 / 12

Start / End Page

  • 3458 - 3462

PubMed ID

  • 17446073

Pubmed Central ID

  • 17446073

International Standard Serial Number (ISSN)

  • 0960-894X

Digital Object Identifier (DOI)

  • 10.1016/j.bmcl.2007.03.081

Language

  • eng

Conference Location

  • England