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Up-regulation of tissue factor in human pulmonary artery endothelial cells after ultrafine particle exposure.

Publication ,  Journal Article
Karoly, ED; Li, Z; Dailey, LA; Hyseni, X; Huang, Y-CT
Published in: Environ Health Perspect
April 2007

BACKGROUND: Epidemiology studies have linked exposure to pollutant particles to increased cardiovascular mortality and morbidity, but the mechanisms remain unknown. OBJECTIVES: We tested the hypothesis that the ultrafine fraction of ambient pollutant particles would cause endothelial cell dysfunction. METHODS: We profiled gene expression of human pulmonary artery endothelial cells (HPAEC) exposed to ultrafine particles (UFPs; 100 microg/mL) from Chapel Hill, North Carolina, or vehicle for 4 hr with Affymetrix HG U133 Plus 2.0 chips (n = 4 each). RESULTS: We found 320 up-regulated genes and 106 down-regulated genes (p < 0.01, 5% false discovery rate). We noted up-regulation of genes related to coagulation [tissue factor (F3) and coagulation factor II receptor-like 2 (F2RL2)] and differential regulation of genes related to F3 signaling (FOS, JUN, and NFKBIA). Results of quantitative polymerase chain reaction show a significant up-regulation of F3 after 10 and 100 microg/mLUFP exposures. Additionally, the water-soluble fractions of UFPs were sufficient to induce the expression of F3, F2RL2, and heme oxygenase 1 (HMOX1). Treatment of HPAEC with UFPs for 16 hr increased the release of interleukin (IL)-6 and IL-8. Pretreatment of HPAEC with a blocking antibody against F3 attenuated IL-6 and IL-8 release by 30 and 70%, respectively. CONCLUSIONS: Using gene profiling, we discovered that UFPs may induce vascular endothelial cells to express genes related to clotting. These results indicate that PM may cause adverse cardiovascular health effects by activating coagulation-inflammation circuitry.

Duke Scholars

Published In

Environ Health Perspect

DOI

ISSN

0091-6765

Publication Date

April 2007

Volume

115

Issue

4

Start / End Page

535 / 540

Location

United States

Related Subject Headings

  • Vehicle Emissions
  • Up-Regulation
  • Toxicology
  • Pulmonary Artery
  • Particle Size
  • Inflammation
  • Humans
  • Gene Expression Profiling
  • Endothelial Cells
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Karoly, E. D., Li, Z., Dailey, L. A., Hyseni, X., & Huang, Y.-C. (2007). Up-regulation of tissue factor in human pulmonary artery endothelial cells after ultrafine particle exposure. Environ Health Perspect, 115(4), 535–540. https://doi.org/10.1289/ehp.9556
Karoly, Edward D., Zhuowei Li, Lisa A. Dailey, Xhevahire Hyseni, and Yuh-Chin T. Huang. “Up-regulation of tissue factor in human pulmonary artery endothelial cells after ultrafine particle exposure.Environ Health Perspect 115, no. 4 (April 2007): 535–40. https://doi.org/10.1289/ehp.9556.
Karoly ED, Li Z, Dailey LA, Hyseni X, Huang Y-CT. Up-regulation of tissue factor in human pulmonary artery endothelial cells after ultrafine particle exposure. Environ Health Perspect. 2007 Apr;115(4):535–40.
Karoly, Edward D., et al. “Up-regulation of tissue factor in human pulmonary artery endothelial cells after ultrafine particle exposure.Environ Health Perspect, vol. 115, no. 4, Apr. 2007, pp. 535–40. Pubmed, doi:10.1289/ehp.9556.
Karoly ED, Li Z, Dailey LA, Hyseni X, Huang Y-CT. Up-regulation of tissue factor in human pulmonary artery endothelial cells after ultrafine particle exposure. Environ Health Perspect. 2007 Apr;115(4):535–540.

Published In

Environ Health Perspect

DOI

ISSN

0091-6765

Publication Date

April 2007

Volume

115

Issue

4

Start / End Page

535 / 540

Location

United States

Related Subject Headings

  • Vehicle Emissions
  • Up-Regulation
  • Toxicology
  • Pulmonary Artery
  • Particle Size
  • Inflammation
  • Humans
  • Gene Expression Profiling
  • Endothelial Cells
  • Cells, Cultured