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Effects of a thioreactive agent, diamide, on neuromuscular transmission in lobster.

Publication ,  Journal Article
Colton, CA; Colton, JS
Published in: Am J Physiol
January 1982

Diamide[diazine-dicarboxylic acid-bis(dimethylamide)], a thiol-oxidizing agent, has both pre- and postsynaptic actions on the glutaminergic neuromuscular junction of the lobster walking leg. Postsynaptically, diamide produced an increase in the response to exogenously applied glutamate, whereas the effect of diamide on presynaptic transmitter release involved two major changes: 1) a decrease in excitatory junction potential amplitude and 2) an increase in miniature junction potential frequency. Short-term facilitation also decreased. Equilibration with 1,4-dithiothreitol (a sulfhydryl-reducing agent) reversed the decline in excitatory junction potential frequency, and the fall in short-term facilitation. The miniature junction potential frequency increase induced by diamide was independent of external Ca2+, as diamide in a Ca2+-free solution produced a similar response to that in a Ca2+-containing solution. We propose that the action of diamide on transmitter release is similar to the action of polyvalent cations, i.e., diamide has two sites of action, a blockade of inward Ca2+ flux and an increased release of Ca2+ inside the terminal.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

January 1982

Volume

242

Issue

1

Start / End Page

C59 / C64

Location

United States

Related Subject Headings

  • Synaptic Transmission
  • Neuromuscular Junction
  • Nephropidae
  • Evoked Potentials
  • Dithiothreitol
  • Diamide
  • Cardiovascular System & Hematology
  • Calcium
  • Azo Compounds
  • Animals
 

Citation

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ICMJE
MLA
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Colton, C. A., & Colton, J. S. (1982). Effects of a thioreactive agent, diamide, on neuromuscular transmission in lobster. Am J Physiol, 242(1), C59–C64. https://doi.org/10.1152/ajpcell.1982.242.1.C59
Colton, C. A., and J. S. Colton. “Effects of a thioreactive agent, diamide, on neuromuscular transmission in lobster.Am J Physiol 242, no. 1 (January 1982): C59–64. https://doi.org/10.1152/ajpcell.1982.242.1.C59.
Colton CA, Colton JS. Effects of a thioreactive agent, diamide, on neuromuscular transmission in lobster. Am J Physiol. 1982 Jan;242(1):C59–64.
Colton, C. A., and J. S. Colton. “Effects of a thioreactive agent, diamide, on neuromuscular transmission in lobster.Am J Physiol, vol. 242, no. 1, Jan. 1982, pp. C59–64. Pubmed, doi:10.1152/ajpcell.1982.242.1.C59.
Colton CA, Colton JS. Effects of a thioreactive agent, diamide, on neuromuscular transmission in lobster. Am J Physiol. 1982 Jan;242(1):C59–C64.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

January 1982

Volume

242

Issue

1

Start / End Page

C59 / C64

Location

United States

Related Subject Headings

  • Synaptic Transmission
  • Neuromuscular Junction
  • Nephropidae
  • Evoked Potentials
  • Dithiothreitol
  • Diamide
  • Cardiovascular System & Hematology
  • Calcium
  • Azo Compounds
  • Animals