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Effect of helium and heliox on glutamate decarboxylase activity.

Publication ,  Journal Article
Colton, JS; Gilman, SC; Chang, P; Colton, CA
Published in: Undersea Biomed Res
July 1990

The activity of glutamate decarboxylase (GAD) in pure helium at 6.8 MPa was significantly enhanced when compared to GAD activity in air at 0.1 MPa (906 vs. 602 nmol.h-1.mg-1 protein, respectively). No significant difference was found between GAD activities in heliox at 6.8 MPa (0.87% O2 at 6.8 MPa) and in air at 0.1 MPa. On the other hand, the activities in heliox at 0.1 MPa (0.87% O2 at 0.1 MPa) and air at 0.1 MPa were significantly different (655 and 446 nmol.h-1.mg-1 protein, respectively). These data indicate that pressures up to 6.8 MPa do not affect the GAD-catalyzed synthesis of gamma-aminobutyric acid, but that the enzyme, as previously reported, is sensitive to low levels of oxygen.

Duke Scholars

Published In

Undersea Biomed Res

ISSN

0093-5387

Publication Date

July 1990

Volume

17

Issue

4

Start / End Page

297 / 303

Location

United States

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Oxygen
  • Neurotransmitter Agents
  • Male
  • In Vitro Techniques
  • High Pressure Neurological Syndrome
  • Helium
  • Guinea Pigs
  • Glutamate Decarboxylase
  • GABA Antagonists
 

Citation

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Colton, J. S., Gilman, S. C., Chang, P., & Colton, C. A. (1990). Effect of helium and heliox on glutamate decarboxylase activity. Undersea Biomed Res, 17(4), 297–303.
Colton, J. S., S. C. Gilman, P. Chang, and C. A. Colton. “Effect of helium and heliox on glutamate decarboxylase activity.Undersea Biomed Res 17, no. 4 (July 1990): 297–303.
Colton JS, Gilman SC, Chang P, Colton CA. Effect of helium and heliox on glutamate decarboxylase activity. Undersea Biomed Res. 1990 Jul;17(4):297–303.
Colton, J. S., et al. “Effect of helium and heliox on glutamate decarboxylase activity.Undersea Biomed Res, vol. 17, no. 4, July 1990, pp. 297–303.
Colton JS, Gilman SC, Chang P, Colton CA. Effect of helium and heliox on glutamate decarboxylase activity. Undersea Biomed Res. 1990 Jul;17(4):297–303.

Published In

Undersea Biomed Res

ISSN

0093-5387

Publication Date

July 1990

Volume

17

Issue

4

Start / End Page

297 / 303

Location

United States

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Oxygen
  • Neurotransmitter Agents
  • Male
  • In Vitro Techniques
  • High Pressure Neurological Syndrome
  • Helium
  • Guinea Pigs
  • Glutamate Decarboxylase
  • GABA Antagonists