Characterization of interleukin-1 production by microglia in culture.

Journal Article (Journal Article)

The production of interleukin-1 (IL-1) by cultured neonatal rat microglia was studied using the D10 cell assay. The results show that IL-1 was secreted in response to lipopolysaccharide (LPS) in a dose- and time-dependent fashion. IL-1 production was specific to microglia and was not induced in astrocytes. Indomethacin, which is known to modulate the release of IL-1 from monocytes, had no effect on LPS-stimulated microglia. Aging of the microglia from two weeks to 4 weeks in culture, however, reduced the release of IL-1 in response to LPS. Our data indicate that microglia are a major source of IL-1 and that the release of IL-1 depends on the presence of inflammatory mediators such as LPS and the age of the culture.

Full Text

Duke Authors

Cited Authors

  • Yao, J; Keri, JE; Taffs, RE; Colton, CA

Published Date

  • September 18, 1992

Published In

Volume / Issue

  • 591 / 1

Start / End Page

  • 88 - 93

PubMed ID

  • 1446236

International Standard Serial Number (ISSN)

  • 0006-8993

Digital Object Identifier (DOI)

  • 10.1016/0006-8993(92)90981-e


  • eng

Conference Location

  • Netherlands