Minimizing pulling geometry errors in atomic force microscope single molecule force spectroscopy.


Journal Article

In atomic force microscopy-based single molecule force spectroscopy (AFM-SMFS), it is assumed that the pulling angle is negligible and that the force applied to the molecule is equivalent to the force measured by the instrument. Recent studies, however, have indicated that the pulling geometry errors can drastically alter the measured force-extension relationship of molecules. Here we describe a software-based alignment method that repositions the cantilever such that it is located directly above the molecule's substrate attachment site. By aligning the applied force with the measurement axis, the molecule is no longer undergoing combined loading, and the full force can be measured by the cantilever. Simulations and experimental results verify the ability of the alignment program to minimize pulling geometry errors in AFM-SMFS studies.

Full Text

Duke Authors

Cited Authors

  • Rivera, M; Lee, W; Ke, C; Marszalek, PE; Cole, DG; Clark, RL

Published Date

  • October 2008

Published In

Volume / Issue

  • 95 / 8

Start / End Page

  • 3991 - 3998

PubMed ID

  • 18641069

Pubmed Central ID

  • 18641069

Electronic International Standard Serial Number (EISSN)

  • 1542-0086

International Standard Serial Number (ISSN)

  • 0006-3495

Digital Object Identifier (DOI)

  • 10.1529/biophysj.108.138842


  • eng