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In situ friction measurement on murine cartilage by atomic force microscopy.

Publication ,  Journal Article
Coles, JM; Blum, JJ; Jay, GD; Darling, EM; Guilak, F; Zauscher, S
Published in: Journal of biomechanics
January 2008

Articular cartilage provides a low-friction, wear-resistant surface for the motion of diarthrodial joints. The objective of this study was to develop a method for in situ friction measurement of murine cartilage using a colloidal probe attached to the cantilever of an atomic force microscope. Sliding friction was measured between a chemically functionalized microsphere and the cartilage of the murine femoral head. Friction was measured at normal loads ranging incrementally from 20 to 100 nN with a sliding speed of 40 microm/s and sliding distance of 64 microm. Under these test conditions, hydrostatic pressurization and biphasic load support in the cartilage were minimized, providing frictional measurements that predominantly reflect boundary lubrication properties. Friction coefficients measured on murine tissue (0.25+/-0.11) were similar to those measured on porcine tissue (0.23+/-0.09) and were in general agreement with measurements of boundary friction on cartilage by other researchers. Using the colloidal probe as an indenter, the elastic mechanical properties and surface roughness were measured in the same configuration. Interfacial shear was found to be the principal mechanism of friction generation, with little to no friction resulting from plowing forces, collision forces, or energy losses due to normal deformation. This measurement technique can be applied to future studies of cartilage friction and mechanical properties on genetically altered mice or other small animals.

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Published In

Journal of biomechanics

DOI

EISSN

1873-2380

ISSN

0021-9290

Publication Date

January 2008

Volume

41

Issue

3

Start / End Page

541 / 548

Related Subject Headings

  • Swine
  • Surface Properties
  • Microscopy, Atomic Force
  • Mice
  • Friction
  • Femur Head
  • Elasticity
  • Cartilage
  • Biomedical Engineering
  • Animals
 

Citation

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ICMJE
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Coles, J. M., Blum, J. J., Jay, G. D., Darling, E. M., Guilak, F., & Zauscher, S. (2008). In situ friction measurement on murine cartilage by atomic force microscopy. Journal of Biomechanics, 41(3), 541–548. https://doi.org/10.1016/j.jbiomech.2007.10.013
Coles, Jeffrey M., Jason J. Blum, Gregory D. Jay, Eric M. Darling, Farshid Guilak, and Stefan Zauscher. “In situ friction measurement on murine cartilage by atomic force microscopy.Journal of Biomechanics 41, no. 3 (January 2008): 541–48. https://doi.org/10.1016/j.jbiomech.2007.10.013.
Coles JM, Blum JJ, Jay GD, Darling EM, Guilak F, Zauscher S. In situ friction measurement on murine cartilage by atomic force microscopy. Journal of biomechanics. 2008 Jan;41(3):541–8.
Coles, Jeffrey M., et al. “In situ friction measurement on murine cartilage by atomic force microscopy.Journal of Biomechanics, vol. 41, no. 3, Jan. 2008, pp. 541–48. Epmc, doi:10.1016/j.jbiomech.2007.10.013.
Coles JM, Blum JJ, Jay GD, Darling EM, Guilak F, Zauscher S. In situ friction measurement on murine cartilage by atomic force microscopy. Journal of biomechanics. 2008 Jan;41(3):541–548.
Journal cover image

Published In

Journal of biomechanics

DOI

EISSN

1873-2380

ISSN

0021-9290

Publication Date

January 2008

Volume

41

Issue

3

Start / End Page

541 / 548

Related Subject Headings

  • Swine
  • Surface Properties
  • Microscopy, Atomic Force
  • Mice
  • Friction
  • Femur Head
  • Elasticity
  • Cartilage
  • Biomedical Engineering
  • Animals