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Global transcriptional and proteomic analysis of the Sig1 heat shock regulon of Deinococcus radiodurans.

Publication ,  Journal Article
Schmid, AK; Howell, HA; Battista, JR; Peterson, SN; Lidstrom, ME
Published in: Journal of bacteriology
May 2005

The sig1 gene, predicted to encode an extracytoplasmic function-type heat shock sigma factor of Deinococcus radiodurans, has been shown to play a central role in the positive regulation of the heat shock operons groESL and dnaKJ. To determine if Sig1 is required for the regulation of additional heat shock genes, we monitored the global transcriptional and proteomic profiles of a D. radiodurans R1 sig1 mutant and wild-type cells in response to elevated temperature stress. Thirty-one gene products were identified that showed heat shock induction in the wild type but not in the sig1 mutant. Quantitative real-time PCR experiments verified the transcriptional requirement of Sig1 for the heat shock induction of the mRNA of five of these genes-dnaK, groES, DR1314, pspA, and hsp20. hsp20 appears to encode a new member of the small heat shock protein superfamily, DR1314 is predicted to encode a hypothetical protein with no recognizable orthologs, and pspA is predicted to encode a protein involved in maintenance of membrane integrity. Deletion mutation analysis demonstrated the importance in heat shock protection of hsp20 and DR1314. The promoters of dnaKJE, groESL, DR1314, pspA, and hsp20 were mapped and, combined with computer-based pattern searches of the upstream regions of the 26 other Sig1 regulon members, these results suggested that Sig1 might recognize both sigma70-type and sigma(W)-type promoter consensus sequences. These results expand the D. radiodurans Sig1 heat shock regulon to include 31 potential new members, including not only factors with cytoplasmic functions, such as groES and dnaK, but also those with extracytoplasmic functions, like pspA.

Duke Scholars

Published In

Journal of bacteriology

DOI

EISSN

1098-5530

ISSN

0021-9193

Publication Date

May 2005

Volume

187

Issue

10

Start / End Page

3339 / 3351

Related Subject Headings

  • Transcription, Genetic
  • Temperature
  • Sigma Factor
  • Reverse Transcriptase Polymerase Chain Reaction
  • Regulon
  • Proteomics
  • Promoter Regions, Genetic
  • Phosphoproteins
  • Mutagenesis
  • Molecular Sequence Data
 

Citation

APA
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ICMJE
MLA
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Schmid, A. K., Howell, H. A., Battista, J. R., Peterson, S. N., & Lidstrom, M. E. (2005). Global transcriptional and proteomic analysis of the Sig1 heat shock regulon of Deinococcus radiodurans. Journal of Bacteriology, 187(10), 3339–3351. https://doi.org/10.1128/jb.187.10.3339-3351.2005
Schmid, Amy K., Heather A. Howell, John R. Battista, Scott N. Peterson, and Mary E. Lidstrom. “Global transcriptional and proteomic analysis of the Sig1 heat shock regulon of Deinococcus radiodurans.Journal of Bacteriology 187, no. 10 (May 2005): 3339–51. https://doi.org/10.1128/jb.187.10.3339-3351.2005.
Schmid AK, Howell HA, Battista JR, Peterson SN, Lidstrom ME. Global transcriptional and proteomic analysis of the Sig1 heat shock regulon of Deinococcus radiodurans. Journal of bacteriology. 2005 May;187(10):3339–51.
Schmid, Amy K., et al. “Global transcriptional and proteomic analysis of the Sig1 heat shock regulon of Deinococcus radiodurans.Journal of Bacteriology, vol. 187, no. 10, May 2005, pp. 3339–51. Epmc, doi:10.1128/jb.187.10.3339-3351.2005.
Schmid AK, Howell HA, Battista JR, Peterson SN, Lidstrom ME. Global transcriptional and proteomic analysis of the Sig1 heat shock regulon of Deinococcus radiodurans. Journal of bacteriology. 2005 May;187(10):3339–3351.

Published In

Journal of bacteriology

DOI

EISSN

1098-5530

ISSN

0021-9193

Publication Date

May 2005

Volume

187

Issue

10

Start / End Page

3339 / 3351

Related Subject Headings

  • Transcription, Genetic
  • Temperature
  • Sigma Factor
  • Reverse Transcriptase Polymerase Chain Reaction
  • Regulon
  • Proteomics
  • Promoter Regions, Genetic
  • Phosphoproteins
  • Mutagenesis
  • Molecular Sequence Data