High-performance liquid chromatography method for quantifying sphingomyelin in rat brain.
Journal Article (Journal Article)
A rapid, reproducible and accurate high-performance liquid chromatographic (HPLC) method for the quantitative determination of sphingomyelin in rat brain was developed and validated using normal-phase silica gel column, acetonitrile-methanol-water (65:18:17 (v/v)) at a flow rate of 1 ml/min, isocratic elution, UV detection at 207 nm and 1,2-dimyristoyl-sn-glycero-3-phosphocholine as an internal standard. Total run time was 10.0 min. The calibration curve was linear over the range of 0.025-0.4 mg/ml sphingomyelin (R2>0.99). The intra-day coefficient of variation ranged from 1.4% to 2.2%. The average inter-day coefficient of variation over a period of 4 days was 3.1%. The practical limit of detection was 0.005 mg/ml with a quantification limit of 0.01 mg/ml.
Full Text
Duke Authors
Cited Authors
- Azzam, DJ; Usta, JA; Mouneimne, Y; El Hokayem, JA; Mikati, MA
Published Date
- November 1, 2007
Published In
Volume / Issue
- 859 / 1
Start / End Page
- 131 - 136
PubMed ID
- 17901003
International Standard Serial Number (ISSN)
- 1570-0232
Digital Object Identifier (DOI)
- 10.1016/j.jchromb.2007.09.005
Language
- eng
Conference Location
- Netherlands