High-performance liquid chromatography method for quantifying sphingomyelin in rat brain.

Journal Article (Journal Article)

A rapid, reproducible and accurate high-performance liquid chromatographic (HPLC) method for the quantitative determination of sphingomyelin in rat brain was developed and validated using normal-phase silica gel column, acetonitrile-methanol-water (65:18:17 (v/v)) at a flow rate of 1 ml/min, isocratic elution, UV detection at 207 nm and 1,2-dimyristoyl-sn-glycero-3-phosphocholine as an internal standard. Total run time was 10.0 min. The calibration curve was linear over the range of 0.025-0.4 mg/ml sphingomyelin (R2>0.99). The intra-day coefficient of variation ranged from 1.4% to 2.2%. The average inter-day coefficient of variation over a period of 4 days was 3.1%. The practical limit of detection was 0.005 mg/ml with a quantification limit of 0.01 mg/ml.

Full Text

Duke Authors

Cited Authors

  • Azzam, DJ; Usta, JA; Mouneimne, Y; El Hokayem, JA; Mikati, MA

Published Date

  • November 1, 2007

Published In

Volume / Issue

  • 859 / 1

Start / End Page

  • 131 - 136

PubMed ID

  • 17901003

International Standard Serial Number (ISSN)

  • 1570-0232

Digital Object Identifier (DOI)

  • 10.1016/j.jchromb.2007.09.005


  • eng

Conference Location

  • Netherlands