Skip to main content
Journal cover image

Human adipocyte lipid-binding protein: purification of the protein and cloning of its complementary DNA.

Publication ,  Journal Article
Baxa, CA; Sha, RS; Buelt, MK; Smith, AJ; Matarese, V; Chinander, LL; Boundy, KL; Bernlohr, DA
Published in: Biochemistry
October 31, 1989

Human adipocyte lipid-binding protein (H-ALBP) was purified from normal subcutaneous adipose tissue to greater than 98% homogeneity, utilizing a combination of acid fractionation, gel filtration, covalent chromatography on activated thiol-Sepharose 4B, and anion-exchange chromatography. Human ALBP comprised about 1% of total cytosolic protein in human adipose tissue, had a relative molecular mass of about 15 kDa, and existed as a monomer in solution. The amino terminus of H-ALBP was blocked to sequencing. When a liposome ligand delivery assay was used, H-ALBP saturably bound oleic acid with about 1 mol of ligand bound per mole of protein. Additionally, H-ALBP saturably bound retinoic acid as determined by the quenching of intrinsic tryptophan fluorescence. A full-length H-ALBP cDNA has been cloned; the sequence predicts a 649-base mRNA comprised of a 62-base 5'-noncoding region containing an 18S ribosome-binding site, a single 396-base open-reading frame, and a 191-base 3'-noncoding region. Comparative sequence analysis indicated that the 132 amino acid H-ALBP is a member of a multigene family of intracellular lipid-binding proteins and contains the consensus substrate phosphorylation sequence for tyrosyl kinases.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

October 31, 1989

Volume

28

Issue

22

Start / End Page

8683 / 8690

Location

United States

Related Subject Headings

  • Tumor Suppressor Proteins
  • Tretinoin
  • RNA
  • Oleic Acids
  • Oleic Acid
  • Neoplasm Proteins
  • Molecular Sequence Data
  • Humans
  • Female
  • Fatty Acid-Binding Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Baxa, C. A., Sha, R. S., Buelt, M. K., Smith, A. J., Matarese, V., Chinander, L. L., … Bernlohr, D. A. (1989). Human adipocyte lipid-binding protein: purification of the protein and cloning of its complementary DNA. Biochemistry, 28(22), 8683–8690. https://doi.org/10.1021/bi00448a003
Baxa, C. A., R. S. Sha, M. K. Buelt, A. J. Smith, V. Matarese, L. L. Chinander, K. L. Boundy, and D. A. Bernlohr. “Human adipocyte lipid-binding protein: purification of the protein and cloning of its complementary DNA.Biochemistry 28, no. 22 (October 31, 1989): 8683–90. https://doi.org/10.1021/bi00448a003.
Baxa CA, Sha RS, Buelt MK, Smith AJ, Matarese V, Chinander LL, et al. Human adipocyte lipid-binding protein: purification of the protein and cloning of its complementary DNA. Biochemistry. 1989 Oct 31;28(22):8683–90.
Baxa, C. A., et al. “Human adipocyte lipid-binding protein: purification of the protein and cloning of its complementary DNA.Biochemistry, vol. 28, no. 22, Oct. 1989, pp. 8683–90. Pubmed, doi:10.1021/bi00448a003.
Baxa CA, Sha RS, Buelt MK, Smith AJ, Matarese V, Chinander LL, Boundy KL, Bernlohr DA. Human adipocyte lipid-binding protein: purification of the protein and cloning of its complementary DNA. Biochemistry. 1989 Oct 31;28(22):8683–8690.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

October 31, 1989

Volume

28

Issue

22

Start / End Page

8683 / 8690

Location

United States

Related Subject Headings

  • Tumor Suppressor Proteins
  • Tretinoin
  • RNA
  • Oleic Acids
  • Oleic Acid
  • Neoplasm Proteins
  • Molecular Sequence Data
  • Humans
  • Female
  • Fatty Acid-Binding Proteins