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Frequency-dependent response of the vascular endothelium to pulsatile shear stress.

Publication ,  Journal Article
Himburg, HA; Dowd, SE; Friedman, MH
Published in: American journal of physiology. Heart and circulatory physiology
July 2007

As a result of the complex blood flow patterns that occur in the arterial tree, certain regions of the vessel wall experience fluctuations in shear stress that are dominated by harmonic frequencies higher than the heart rate (11). To assess whether variations in frequency affect endothelial gene expression, the gene expression patterns of cultured porcine aortic endothelium exposed to three sinusoidal waveforms (1, 2, and 3 Hz; amplitude = 15 dyn/cm(2)) and one physiological waveform were compared with the expression profiles elicited by steady flow. At each frequency, including steady flow, three levels of mean shear stress (0, 7.5, and 15 dyn/cm(2)) were used. After 24 h shear exposure, RNA was extracted for microarray analysis against 10,665 Sus scrofa oligonucleotides. A two-way ANOVA identified 232 genes of which their transcription was differentially modulated by frequency, while mean shear significantly affected the expression of approximately 3,000 genes. One-way ANOVAs showed that the number of frequency-dependent genes increased as the mean shear stress was reduced. At 1 Hz, several inflammatory transcripts were repressed relative to steady flow, including VCAM and IL-8, whereas several atheroprotective transcripts were induced. The anti-inflammatory response at 1 Hz was reversed at 2 Hz. The proinflammatory response evoked by the higher frequency was most pronounced under reversing and oscillatory shear. This study suggests that arterial regions subject to both shear reversal and dominant frequencies that exceed the normal heart rate are at greater risk for atherosclerotic lesion development.

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Published In

American journal of physiology. Heart and circulatory physiology

DOI

EISSN

1522-1539

ISSN

0363-6135

Publication Date

July 2007

Volume

293

Issue

1

Start / End Page

H645 / H653

Related Subject Headings

  • Swine
  • Shear Strength
  • Pulsatile Flow
  • Proteome
  • Mechanotransduction, Cellular
  • In Vitro Techniques
  • Gene Expression Regulation
  • Endothelial Cells
  • Cells, Cultured
  • Cardiovascular System & Hematology
 

Citation

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MLA
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Himburg, H. A., Dowd, S. E., & Friedman, M. H. (2007). Frequency-dependent response of the vascular endothelium to pulsatile shear stress. American Journal of Physiology. Heart and Circulatory Physiology, 293(1), H645–H653. https://doi.org/10.1152/ajpheart.01087.2006
Himburg, Heather A., Scot E. Dowd, and Morton H. Friedman. “Frequency-dependent response of the vascular endothelium to pulsatile shear stress.American Journal of Physiology. Heart and Circulatory Physiology 293, no. 1 (July 2007): H645–53. https://doi.org/10.1152/ajpheart.01087.2006.
Himburg HA, Dowd SE, Friedman MH. Frequency-dependent response of the vascular endothelium to pulsatile shear stress. American journal of physiology Heart and circulatory physiology. 2007 Jul;293(1):H645–53.
Himburg, Heather A., et al. “Frequency-dependent response of the vascular endothelium to pulsatile shear stress.American Journal of Physiology. Heart and Circulatory Physiology, vol. 293, no. 1, July 2007, pp. H645–53. Epmc, doi:10.1152/ajpheart.01087.2006.
Himburg HA, Dowd SE, Friedman MH. Frequency-dependent response of the vascular endothelium to pulsatile shear stress. American journal of physiology Heart and circulatory physiology. 2007 Jul;293(1):H645–H653.

Published In

American journal of physiology. Heart and circulatory physiology

DOI

EISSN

1522-1539

ISSN

0363-6135

Publication Date

July 2007

Volume

293

Issue

1

Start / End Page

H645 / H653

Related Subject Headings

  • Swine
  • Shear Strength
  • Pulsatile Flow
  • Proteome
  • Mechanotransduction, Cellular
  • In Vitro Techniques
  • Gene Expression Regulation
  • Endothelial Cells
  • Cells, Cultured
  • Cardiovascular System & Hematology