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Individual and combined effects of shear stress magnitude and spatial gradient on endothelial cell gene expression

Publication ,  Journal Article
Lamack, JA; Friedman, MH
Published in: American Journal Of Physiology-heart And Circulatory Physiology
November 2007

Individual and combined effects of shear stress magnitude and spatial gradient on endothelial cell gene expression. Am J Physiol Heart Circ Physiol 293: H2853-H2859, 2007. First published August 31, 2007; doi:10.1152/ajpheart.00244.2007. The apparent tendency of atherosclerotic lesions to form in complex blood flow environments has led to many theories regarding the importance of hemodynamic forces in endothelium-mediated atherosusceptibility. The effects of shear stress magnitude and spatial shear stress gradient on endothelial cell gene expression in vitro were examined in this study. Converging-width flow channels were designed to impose physiological ranges of shear stress gradient and magnitude on porcine aortic endothelial cells, and real-time quantitative PCR was performed to evaluate their expression of five genes of interest. Although vascular cell adhesion molecule-1 expression was insensitive to either variable, each of the remaining genes exhibited a unique dependence on shear stress magnitude and gradient. Endothelial nitric oxide synthase showed a strong positive dependence on magnitude but was insensitive to gradient. The expression of c-jun was weakly correlated with magnitude and gradient, without an interaction effect. Monocyte chemoattractant protein-1 expression varied inversely with gradient and also depended on the interaction of gradient with magnitude. Intercellular adhesion molecule-1 expression also exhibited an interaction effect, and increased with shear magnitude. These results support the notion that vascular endothelial cells are able to sense shear gradient and magnitude independently.

Duke Scholars

Published In

American Journal Of Physiology-heart And Circulatory Physiology

ISSN

0363-6135

Publication Date

November 2007

Volume

293

Issue

5

Start / End Page

H2853 / H2859

Related Subject Headings

  • Swine
  • Stress, Mechanical
  • Shear Strength
  • Models, Cardiovascular
  • Mechanotransduction, Cellular
  • Immunologic Factors
  • Gene Expression Regulation
  • Endothelial Cells
  • Cytokines
  • Computer Simulation
 

Citation

APA
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ICMJE
MLA
NLM
Lamack, J. A., & Friedman, M. H. (2007). Individual and combined effects of shear stress magnitude and spatial gradient on endothelial cell gene expression. American Journal Of Physiology-Heart And Circulatory Physiology, 293(5), H2853–H2859.
Lamack, J. A., and M. H. Friedman. “Individual and combined effects of shear stress magnitude and spatial gradient on endothelial cell gene expression.” American Journal Of Physiology-Heart And Circulatory Physiology 293, no. 5 (November 2007): H2853–59.
Lamack JA, Friedman MH. Individual and combined effects of shear stress magnitude and spatial gradient on endothelial cell gene expression. American Journal Of Physiology-heart And Circulatory Physiology. 2007 Nov;293(5):H2853–9.
Lamack, J. A., and M. H. Friedman. “Individual and combined effects of shear stress magnitude and spatial gradient on endothelial cell gene expression.” American Journal Of Physiology-Heart And Circulatory Physiology, vol. 293, no. 5, Nov. 2007, pp. H2853–59.
Lamack JA, Friedman MH. Individual and combined effects of shear stress magnitude and spatial gradient on endothelial cell gene expression. American Journal Of Physiology-heart And Circulatory Physiology. 2007 Nov;293(5):H2853–H2859.

Published In

American Journal Of Physiology-heart And Circulatory Physiology

ISSN

0363-6135

Publication Date

November 2007

Volume

293

Issue

5

Start / End Page

H2853 / H2859

Related Subject Headings

  • Swine
  • Stress, Mechanical
  • Shear Strength
  • Models, Cardiovascular
  • Mechanotransduction, Cellular
  • Immunologic Factors
  • Gene Expression Regulation
  • Endothelial Cells
  • Cytokines
  • Computer Simulation