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Zonal changes in the three-dimensional morphology of the chondron under compression: The relationship among cellular, pericellular, and extracellular deformation in articular cartilage

Publication ,  Journal Article
Choi, JB; Youn, I; Cao, L; Leddy, HA; Gilchrist, CL; Setton, LA; Guilak, F
Published in: Journal Of Biomechanics
2007

The pericellular matrix (PCM) is a narrow region of tissue that completely surrounds chondrocytes in articular cartilage. Previous theoretical models of the "chondron" (the PCM with enclosed cells) suggest that the structure and properties of the PCM may significantly influence the mechanical environment of the chondrocyte. The objective of this study was to quantify changes in the three-dimensional (31) morphology of the chondron in situ at different magnitudes of compression applied to the cartilage extracellular matrix. Fluorescence immunolabeling for type-VI collagen was used to identify the boundaries of the cell and PCM, and confocal microscopy was used to form 3D images of chondrons from superficial, middle, and deep zone cartilage in explants compressed to 0\%, 10\%, 30\%, and 50\% surface-to-surface strain. Lagrangian tissue strain, determined locally using texture correlation, was highly inhomogeneous and revealed depth-dependent compressive stiffness and Poisson's ratio of the extracellular matrix. Compression significantly decreased cell and chondron height and volume, depending on the zone and magnitude of compression. In the superficial zone, cellular-level strains were always lower than tissue-level strains. In the middle and deep zones, however, tissue strains below 25\% were amplified at the cellular level, while tissue strains above 25\% were decreased at the cellular level. These findings are consistent with previous theoretical models of the chondron, suggesting that the PCM can serve as either a protective layer for the chondrocyte or a transducer that amplifies strain, such that cellular-level strains are more homogenous throughout the tissue depth despite large inhomogeneities in local ECM strains. (c) 2007 Elsevier Ltd. All rights reserved.

Duke Scholars

Published In

Journal Of Biomechanics

ISSN

0021-9290

Publication Date

2007

Volume

40

Issue

12

Start / End Page

2596 / 2603

Related Subject Headings

  • Weight-Bearing
  • Swine
  • Models, Biological
  • Imaging, Three-Dimensional
  • Female
  • Extracellular Matrix
  • Compressive Strength
  • Collagen Type IV
  • Chondrocytes
  • Cartilage, Articular
 

Citation

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Choi, J. B., Youn, I., Cao, L., Leddy, H. A., Gilchrist, C. L., Setton, L. A., & Guilak, F. (2007). Zonal changes in the three-dimensional morphology of the chondron under compression: The relationship among cellular, pericellular, and extracellular deformation in articular cartilage. Journal Of Biomechanics, 40(12), 2596–2603.
Choi, J. B., I. Youn, L. Cao, H. A. Leddy, C. L. Gilchrist, L. A. Setton, and F. Guilak. “Zonal changes in the three-dimensional morphology of the chondron under compression: The relationship among cellular, pericellular, and extracellular deformation in articular cartilage.” Journal Of Biomechanics 40, no. 12 (2007): 2596–2603.
Journal cover image

Published In

Journal Of Biomechanics

ISSN

0021-9290

Publication Date

2007

Volume

40

Issue

12

Start / End Page

2596 / 2603

Related Subject Headings

  • Weight-Bearing
  • Swine
  • Models, Biological
  • Imaging, Three-Dimensional
  • Female
  • Extracellular Matrix
  • Compressive Strength
  • Collagen Type IV
  • Chondrocytes
  • Cartilage, Articular