SERS-based plasmonic nanobiosensing in single living cells.

Journal Article (Journal Article)

In this paper, we describe the development and application of a pH-sensitive plasmonics-active fiber-optic nanoprobe suitable for intracellular bioanalysis in single living human cells using surface-enhanced Raman scattering (SERS) detection. The effectiveness and usefulness of SERS-based fiber-optic nanoprobes are illustrated by measurements of intracellular pH in HMEC-15/hTERT immortalized "normal" human mammary epithelial cells and PC-3 human prostate cancer cells. The results indicate that fiber-optic nanoprobe insertion and interrogation provide a sensitive and selective means to monitor cellular microenvironments at the single cell level.

Full Text

Duke Authors

Cited Authors

  • Scaffidi, JP; Gregas, MK; Seewaldt, V; Vo-Dinh, T

Published Date

  • February 2009

Published In

Volume / Issue

  • 393 / 4

Start / End Page

  • 1135 - 1141

PubMed ID

  • 19066865

Pubmed Central ID

  • PMC4022298

Electronic International Standard Serial Number (EISSN)

  • 1618-2650

International Standard Serial Number (ISSN)

  • 1618-2642

Digital Object Identifier (DOI)

  • 10.1007/s00216-008-2521-y


  • eng