Intensified biochip system using chemiluminescence for the detection of Bacillus globigii spores.
Journal Article
This paper reports the first intensified biochip system for chemiluminescence detection and the feasibility of using this system for the analysis of biological warfare agents is demonstrated. An enzyme-linked immunosorbent assay targeting Bacillus globigii spores, a surrogate species for Bacillus anthracis, using a chemiluminescent alkaline phosphatase substrate is combined with a compact intensified biochip detection system. The enzymatic amplification was found to be an attractive method for detection of low spore concentrations when combined with the intensified biochip device. This system was capable of detecting approximately 1 x 10(5) Bacillus globigii spores. Moreover, the chemiluminescence method, combined with the self-contained biochip design, allows for a simple, compact system that does not require laser excitation and is readily adaptable to field use.
Full Text
Duke Authors
Cited Authors
- Stratis-Cullum, DN; Griffin, GD; Mobley, J; Vo-Dinh, T
Published Date
- July 2008
Published In
Volume / Issue
- 391 / 5
Start / End Page
- 1655 - 1660
PubMed ID
- 18224472
Pubmed Central ID
- 18224472
Electronic International Standard Serial Number (EISSN)
- 1618-2650
International Standard Serial Number (ISSN)
- 1618-2642
Digital Object Identifier (DOI)
- 10.1007/s00216-008-1835-0
Language
- eng